Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants

Fu Wen Zhou, Zi Qiang Luo, Dan Dan Feng, Cha Xiang Guan, Chang Qing Zhang, Xiao Qun Qin, Xiu Hong Sun

Research output: Contribution to journalArticle

Abstract

AIM: To investigate the effects of low concentrations (1 × 10-13 ∼ 1 × 10-9 mol·L-1) endothelin-1 (ET-1) on the activity of CTP-phosphorylcholine cytidylyltransferase (CCT) from cultured lung explants without serum. METHODS: CDP-[M-14C] choline content as indicator of CCT activity was measured by liquid scintillation. RESULTS: (1) The production of CDP-[M-14C] choline from lung tissue microsomal CCT and cytosolic CCT was dependant on length of reaction time and the content of protein; (2) ET-1 increased lung tissue microsomal CCT activity in a dose-dependent and time-dependent manner; (3) ET-1 (1 × 10-1 mol·L-1) enhanced lung tissue microsomal CCT activity and decreased cytosolic CCT activity; (4) The stimulatory effect of ET-1 (1 × 10 - 10 mol·L-1) on the microsomal CCT activity was abolished by protein kinase C (PKC) inhibitor H-7 and calmodulin antagonist W-7 respectively. CONCLUSION: ET-1 at lower concentrations can activate CCT by translocation of enzyme from cytosol to microsomes and increase lung tissue microsomal CCT activity, which are mediated by PKC and calmodulin.

Original languageEnglish (US)
Pages (from-to)382-385
Number of pages4
JournalChinese Pharmacological Bulletin
Volume18
Issue number4
StatePublished - Jan 1 2002

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Cytidine Triphosphate
Phosphorylcholine
Endothelin-1
Lung
Cytidine Diphosphate
Calmodulin
Choline
Protein Kinase C
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein C Inhibitor
Protein Kinase Inhibitors
Microsomes
Cytosol

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Zhou, F. W., Luo, Z. Q., Feng, D. D., Guan, C. X., Zhang, C. Q., Qin, X. Q., & Sun, X. H. (2002). Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants. Chinese Pharmacological Bulletin, 18(4), 382-385.

Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants. / Zhou, Fu Wen; Luo, Zi Qiang; Feng, Dan Dan; Guan, Cha Xiang; Zhang, Chang Qing; Qin, Xiao Qun; Sun, Xiu Hong.

In: Chinese Pharmacological Bulletin, Vol. 18, No. 4, 01.01.2002, p. 382-385.

Research output: Contribution to journalArticle

Zhou, FW, Luo, ZQ, Feng, DD, Guan, CX, Zhang, CQ, Qin, XQ & Sun, XH 2002, 'Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants', Chinese Pharmacological Bulletin, vol. 18, no. 4, pp. 382-385.
Zhou FW, Luo ZQ, Feng DD, Guan CX, Zhang CQ, Qin XQ et al. Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants. Chinese Pharmacological Bulletin. 2002 Jan 1;18(4):382-385.
Zhou, Fu Wen ; Luo, Zi Qiang ; Feng, Dan Dan ; Guan, Cha Xiang ; Zhang, Chang Qing ; Qin, Xiao Qun ; Sun, Xiu Hong. / Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants. In: Chinese Pharmacological Bulletin. 2002 ; Vol. 18, No. 4. pp. 382-385.
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