Effects of endothelins on collagen turnover in cardiac fibroblasts

Eduardo Guarda, Laxmansa C. Katwa, Paul R. Myers, Suresh C. Tyagi, Karl Weber

Research output: Contribution to journalArticle

Abstract

Objectives: Endothelins (ET-1 and ET-3) may be promoters of tissue remodelling, including fibrillar collagen accumulation. The hypothesis that ET-1 and ET-3 each modify cardiac fibroblast collagen turnover was tested. Methods: Confluent adult rat cardiac fibroblasts were maintained for 24 hours in medium with 0.4% fetal call serum, and varying concentrations of ET-1 or ET-3. Collagen synthesis was measured by 3H-proline incorporation. The synthesis of type I and III collagens was measured by enzyme linked immunosorbent assay (ELISA). The effects of endothelins on collagenase activity were estimated by zymography. Results: ET-1 and ET-3 increased collagen synthesis in a concentration-dependent manner with a maximal 1.7-fold increase (p < 0.001 v control cells). The effects of ET-1 or ET-3 on collagen synthesis were not blocked by PED-3512-PI (10-6 M). an ETA receptor antagonist. ET-1 and ET-3 each significantly (p < 0.01) increased the synthesis of both type I and III collagens. ET-1 caused a 5.8-fold decrease in collagenase activity (p<0.01 v control), and this effect was blocked by PED-3512-PI (10-6 M). ET-3 did not alter collagenase activity. Conclusion: ET-1 and ET-3 increased the synthesis of type I and III collagens, whereas ET-1. but not ET-3, reduced collagenase activity. The effects of endothelins on collagen synthesis in cardiac fibroblasts seem to be mediated through ETA and ETB receptors, whereas their effects on collagenase seem to be mediated by ETA receptors.Cardiovascular Research 1993: 27: 2130-2134.

Original languageEnglish (US)
Pages (from-to)2130-2134
Number of pages5
JournalCardiovascular research
Volume27
Issue number12
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Endothelins
Collagenases
Collagen
Fibroblasts
Collagen Type III
Collagen Type I
Fibrillar Collagens
Matrix Metalloproteinase 13
Endothelin-1
Proline
Enzyme-Linked Immunosorbent Assay
Serum
Research

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Effects of endothelins on collagen turnover in cardiac fibroblasts. / Guarda, Eduardo; Katwa, Laxmansa C.; Myers, Paul R.; Tyagi, Suresh C.; Weber, Karl.

In: Cardiovascular research, Vol. 27, No. 12, 01.01.1993, p. 2130-2134.

Research output: Contribution to journalArticle

Guarda, Eduardo ; Katwa, Laxmansa C. ; Myers, Paul R. ; Tyagi, Suresh C. ; Weber, Karl. / Effects of endothelins on collagen turnover in cardiac fibroblasts. In: Cardiovascular research. 1993 ; Vol. 27, No. 12. pp. 2130-2134.
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abstract = "Objectives: Endothelins (ET-1 and ET-3) may be promoters of tissue remodelling, including fibrillar collagen accumulation. The hypothesis that ET-1 and ET-3 each modify cardiac fibroblast collagen turnover was tested. Methods: Confluent adult rat cardiac fibroblasts were maintained for 24 hours in medium with 0.4{\%} fetal call serum, and varying concentrations of ET-1 or ET-3. Collagen synthesis was measured by 3H-proline incorporation. The synthesis of type I and III collagens was measured by enzyme linked immunosorbent assay (ELISA). The effects of endothelins on collagenase activity were estimated by zymography. Results: ET-1 and ET-3 increased collagen synthesis in a concentration-dependent manner with a maximal 1.7-fold increase (p < 0.001 v control cells). The effects of ET-1 or ET-3 on collagen synthesis were not blocked by PED-3512-PI (10-6 M). an ETA receptor antagonist. ET-1 and ET-3 each significantly (p < 0.01) increased the synthesis of both type I and III collagens. ET-1 caused a 5.8-fold decrease in collagenase activity (p<0.01 v control), and this effect was blocked by PED-3512-PI (10-6 M). ET-3 did not alter collagenase activity. Conclusion: ET-1 and ET-3 increased the synthesis of type I and III collagens, whereas ET-1. but not ET-3, reduced collagenase activity. The effects of endothelins on collagen synthesis in cardiac fibroblasts seem to be mediated through ETA and ETB receptors, whereas their effects on collagenase seem to be mediated by ETA receptors.Cardiovascular Research 1993: 27: 2130-2134.",
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