Effects of exercise and obesity on UCP3 content in Rat Hindlimb Muscles

Jonathan M. Peterson, Randall W. Bryner, Jefferson C. Frisbee, Stephen Alway

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Uncoupling protein 3 (UCP3) is a mitochondrial inner membrane protein, which is hypothesized to shuttle nonmetabolized fatty acids, particularly when excessive fatty acids are present. Purpose: Obese Zucker rats (OZR) have systematically elevated levels of fatty acids, with decreased fatty acid metabolism. We hypothesized that basal UCP3 protein expression levels would be elevated in the skeletal muscles of the OZR compared with the lean Zucker rats (LZR). In addition, because aerobic exercise training has been shown to elevate the ability of skeletal muscle to metabolize lipids, we also hypothesized that aerobic exercise training would decrease skeletal muscle UCP3 protein expression and that this would be more pronounced in the skeletal muscles of the OZR. Methods: OZR and LZR were aerobically trained on a motorized treadmill for 55 mind -1, 5 dwk -1, for 9 wk. UCP3 and oxidative enzymes were measured in plantaris, gastrocnemius, and soleus muscles. Results: Basal UCP3 protein expression was elevated approximately eightfold in the plantaris muscles and threefold in the gastrocnemius muscles of the OZR compared with the LZR (P < 0.05). However, there was no difference in UCP3 protein expression in the soleus muscles of the OZR compared with the LZR (P = 0.34). Furthermore, aerobic exercise training did not significantly alter UCP3 protein expression in the soleus, plantaris, or gastrocnemius muscles of the LZR; however, UCP3 protein expression levels decreased in trained OZR soleus and gastrocnemius muscles compared with controls. Conclusions: The decrease in UCP3 with aerobic exercise training was most notable in the soleus of the OZR. These data demonstrate that the exercise-induced adaptations of UCP3 protein levels are muscle specific in obese animals compared with lean animals.

Original languageEnglish (US)
Pages (from-to)1616-1622
Number of pages7
JournalMedicine and Science in Sports and Exercise
Volume40
Issue number9
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Zucker Rats
Hindlimb
Skeletal Muscle
Obesity
Muscles
Exercise
Fatty Acids
Proteins
Muscle Proteins
Uncoupling Protein 3

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of exercise and obesity on UCP3 content in Rat Hindlimb Muscles. / Peterson, Jonathan M.; Bryner, Randall W.; Frisbee, Jefferson C.; Alway, Stephen.

In: Medicine and Science in Sports and Exercise, Vol. 40, No. 9, 01.12.2008, p. 1616-1622.

Research output: Contribution to journalArticle

Peterson, Jonathan M. ; Bryner, Randall W. ; Frisbee, Jefferson C. ; Alway, Stephen. / Effects of exercise and obesity on UCP3 content in Rat Hindlimb Muscles. In: Medicine and Science in Sports and Exercise. 2008 ; Vol. 40, No. 9. pp. 1616-1622.
@article{d8d46feb7ad24c6a8bad5ecf0d7b5b49,
title = "Effects of exercise and obesity on UCP3 content in Rat Hindlimb Muscles",
abstract = "Uncoupling protein 3 (UCP3) is a mitochondrial inner membrane protein, which is hypothesized to shuttle nonmetabolized fatty acids, particularly when excessive fatty acids are present. Purpose: Obese Zucker rats (OZR) have systematically elevated levels of fatty acids, with decreased fatty acid metabolism. We hypothesized that basal UCP3 protein expression levels would be elevated in the skeletal muscles of the OZR compared with the lean Zucker rats (LZR). In addition, because aerobic exercise training has been shown to elevate the ability of skeletal muscle to metabolize lipids, we also hypothesized that aerobic exercise training would decrease skeletal muscle UCP3 protein expression and that this would be more pronounced in the skeletal muscles of the OZR. Methods: OZR and LZR were aerobically trained on a motorized treadmill for 55 mind -1, 5 dwk -1, for 9 wk. UCP3 and oxidative enzymes were measured in plantaris, gastrocnemius, and soleus muscles. Results: Basal UCP3 protein expression was elevated approximately eightfold in the plantaris muscles and threefold in the gastrocnemius muscles of the OZR compared with the LZR (P < 0.05). However, there was no difference in UCP3 protein expression in the soleus muscles of the OZR compared with the LZR (P = 0.34). Furthermore, aerobic exercise training did not significantly alter UCP3 protein expression in the soleus, plantaris, or gastrocnemius muscles of the LZR; however, UCP3 protein expression levels decreased in trained OZR soleus and gastrocnemius muscles compared with controls. Conclusions: The decrease in UCP3 with aerobic exercise training was most notable in the soleus of the OZR. These data demonstrate that the exercise-induced adaptations of UCP3 protein levels are muscle specific in obese animals compared with lean animals.",
author = "Peterson, {Jonathan M.} and Bryner, {Randall W.} and Frisbee, {Jefferson C.} and Stephen Alway",
year = "2008",
month = "12",
day = "1",
doi = "10.1249/MSS.0b013e31817702a4",
language = "English (US)",
volume = "40",
pages = "1616--1622",
journal = "Medicine and Science in Sports and Exercise",
issn = "0195-9131",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Effects of exercise and obesity on UCP3 content in Rat Hindlimb Muscles

AU - Peterson, Jonathan M.

AU - Bryner, Randall W.

AU - Frisbee, Jefferson C.

AU - Alway, Stephen

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Uncoupling protein 3 (UCP3) is a mitochondrial inner membrane protein, which is hypothesized to shuttle nonmetabolized fatty acids, particularly when excessive fatty acids are present. Purpose: Obese Zucker rats (OZR) have systematically elevated levels of fatty acids, with decreased fatty acid metabolism. We hypothesized that basal UCP3 protein expression levels would be elevated in the skeletal muscles of the OZR compared with the lean Zucker rats (LZR). In addition, because aerobic exercise training has been shown to elevate the ability of skeletal muscle to metabolize lipids, we also hypothesized that aerobic exercise training would decrease skeletal muscle UCP3 protein expression and that this would be more pronounced in the skeletal muscles of the OZR. Methods: OZR and LZR were aerobically trained on a motorized treadmill for 55 mind -1, 5 dwk -1, for 9 wk. UCP3 and oxidative enzymes were measured in plantaris, gastrocnemius, and soleus muscles. Results: Basal UCP3 protein expression was elevated approximately eightfold in the plantaris muscles and threefold in the gastrocnemius muscles of the OZR compared with the LZR (P < 0.05). However, there was no difference in UCP3 protein expression in the soleus muscles of the OZR compared with the LZR (P = 0.34). Furthermore, aerobic exercise training did not significantly alter UCP3 protein expression in the soleus, plantaris, or gastrocnemius muscles of the LZR; however, UCP3 protein expression levels decreased in trained OZR soleus and gastrocnemius muscles compared with controls. Conclusions: The decrease in UCP3 with aerobic exercise training was most notable in the soleus of the OZR. These data demonstrate that the exercise-induced adaptations of UCP3 protein levels are muscle specific in obese animals compared with lean animals.

AB - Uncoupling protein 3 (UCP3) is a mitochondrial inner membrane protein, which is hypothesized to shuttle nonmetabolized fatty acids, particularly when excessive fatty acids are present. Purpose: Obese Zucker rats (OZR) have systematically elevated levels of fatty acids, with decreased fatty acid metabolism. We hypothesized that basal UCP3 protein expression levels would be elevated in the skeletal muscles of the OZR compared with the lean Zucker rats (LZR). In addition, because aerobic exercise training has been shown to elevate the ability of skeletal muscle to metabolize lipids, we also hypothesized that aerobic exercise training would decrease skeletal muscle UCP3 protein expression and that this would be more pronounced in the skeletal muscles of the OZR. Methods: OZR and LZR were aerobically trained on a motorized treadmill for 55 mind -1, 5 dwk -1, for 9 wk. UCP3 and oxidative enzymes were measured in plantaris, gastrocnemius, and soleus muscles. Results: Basal UCP3 protein expression was elevated approximately eightfold in the plantaris muscles and threefold in the gastrocnemius muscles of the OZR compared with the LZR (P < 0.05). However, there was no difference in UCP3 protein expression in the soleus muscles of the OZR compared with the LZR (P = 0.34). Furthermore, aerobic exercise training did not significantly alter UCP3 protein expression in the soleus, plantaris, or gastrocnemius muscles of the LZR; however, UCP3 protein expression levels decreased in trained OZR soleus and gastrocnemius muscles compared with controls. Conclusions: The decrease in UCP3 with aerobic exercise training was most notable in the soleus of the OZR. These data demonstrate that the exercise-induced adaptations of UCP3 protein levels are muscle specific in obese animals compared with lean animals.

UR - http://www.scopus.com/inward/record.url?scp=66149099634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66149099634&partnerID=8YFLogxK

U2 - 10.1249/MSS.0b013e31817702a4

DO - 10.1249/MSS.0b013e31817702a4

M3 - Article

VL - 40

SP - 1616

EP - 1622

JO - Medicine and Science in Sports and Exercise

JF - Medicine and Science in Sports and Exercise

SN - 0195-9131

IS - 9

ER -