Effects of high-protein versus high-carbohydrate diets on markers of β- Cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in obese, premenopausal women without diabetes

Abbas E. Kitabchi, Kristin A. McDaniel, Jim Wan, Frances Tylavsky, Crystal A. Jacovino, Chris W. Sands, Ebenezer Nyenwe, Frankie B. Stentz

Research output: Contribution to journalArticle

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Abstract

Objective-To study the effects of high-protein versus high-carbohydrate diets on various metabolic end points (glucoregulation, oxidative stress [dichlorofluorescein], lipid peroxidation [malondialdehyde], proinflammatory cytokines [tumor necrosis factor-α and interleukin-6], adipokines, and resting energy expenditure [REE]) with high protein-low carbohydrate (HP) and high carbohydrate-low protein (HC) diets at baseline and after 6 months of dietary intervention. Research design and methods-We recruited obese, premenopausal women aged 20-50 years with no diabetes or prediabetes who were randomized to HC (55% carbohydrates, 30%fat, and 15%protein) or HP (40% carbohydrates, 30%fat, and 30%protein) diets for 6 months. The diets were provided in prepackaged food, which provided 500 kcal restrictions per day. The above metabolic end points were measured with HP and HC diet at baseline and after 6 months of dietary intervention. Results-After 6months of the HP versus HC diet (12 in each group), the following changes were significantly different by Wilcoxon rank sum test for the following parameters: dichloro-fluorescein (-0.8 vs. -0.3 μmol/L, P < 0.0001), malondialdehyDe (-0.4 vs.-0.2 mmol/L, P = 0.0004), C-reactive protein (-2.1 vs.-0.8mg/L, P = 0.0003), E-selectin (-8.6 vs.-3.7 ng/mL, P = 0.0007), adiponectin (1,284 vs. 504 ng/mL, P = 0.0011), tumor necrosis factor-α (-1.8 vs. -0.9 pg/mL, P < 0.0001), IL-6 (-1.3 vs. -0.4 pg/mL, P < 0.0001), free fatty acid (-0.12 vs. 0.16 mmol/L, P = 0.0002), REE (259 vs. 26 kcal, P < 0.0001), insulin sensitivity (4 vs. 0.9, P < 0.0001), and β- cell function (7.4 vs. 2.1, P < 0.0001). Conclusions-To our knowledge, this is the first report on the significant advantages of a 6-month hypocaloric HP diet versus hypocaloric HC diet on markers of β- cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in normal, obese females without diabetes.

Original languageEnglish (US)
Pages (from-to)1919-1925
Number of pages7
JournalDiabetes care
Volume36
Issue number7
DOIs
StatePublished - Oct 28 2013

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Adipokines
Lipid Peroxidation
Oxidative Stress
Carbohydrates
Cytokines
Diet
Protein-Restricted Diet
Proteins
Nonparametric Statistics
Malondialdehyde
Energy Metabolism
Interleukin-6
Tumor Necrosis Factor-alpha
Fats
Carbohydrate-Restricted Diet
Prediabetic State
E-Selectin
Adiponectin
Fluorescein
Nonesterified Fatty Acids

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Advanced and Specialized Nursing

Cite this

Effects of high-protein versus high-carbohydrate diets on markers of β- Cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in obese, premenopausal women without diabetes. / Kitabchi, Abbas E.; McDaniel, Kristin A.; Wan, Jim; Tylavsky, Frances; Jacovino, Crystal A.; Sands, Chris W.; Nyenwe, Ebenezer; Stentz, Frankie B.

In: Diabetes care, Vol. 36, No. 7, 28.10.2013, p. 1919-1925.

Research output: Contribution to journalArticle

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title = "Effects of high-protein versus high-carbohydrate diets on markers of β- Cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in obese, premenopausal women without diabetes",
abstract = "Objective-To study the effects of high-protein versus high-carbohydrate diets on various metabolic end points (glucoregulation, oxidative stress [dichlorofluorescein], lipid peroxidation [malondialdehyde], proinflammatory cytokines [tumor necrosis factor-α and interleukin-6], adipokines, and resting energy expenditure [REE]) with high protein-low carbohydrate (HP) and high carbohydrate-low protein (HC) diets at baseline and after 6 months of dietary intervention. Research design and methods-We recruited obese, premenopausal women aged 20-50 years with no diabetes or prediabetes who were randomized to HC (55{\%} carbohydrates, 30{\%}fat, and 15{\%}protein) or HP (40{\%} carbohydrates, 30{\%}fat, and 30{\%}protein) diets for 6 months. The diets were provided in prepackaged food, which provided 500 kcal restrictions per day. The above metabolic end points were measured with HP and HC diet at baseline and after 6 months of dietary intervention. Results-After 6months of the HP versus HC diet (12 in each group), the following changes were significantly different by Wilcoxon rank sum test for the following parameters: dichloro-fluorescein (-0.8 vs. -0.3 μmol/L, P < 0.0001), malondialdehyDe (-0.4 vs.-0.2 mmol/L, P = 0.0004), C-reactive protein (-2.1 vs.-0.8mg/L, P = 0.0003), E-selectin (-8.6 vs.-3.7 ng/mL, P = 0.0007), adiponectin (1,284 vs. 504 ng/mL, P = 0.0011), tumor necrosis factor-α (-1.8 vs. -0.9 pg/mL, P < 0.0001), IL-6 (-1.3 vs. -0.4 pg/mL, P < 0.0001), free fatty acid (-0.12 vs. 0.16 mmol/L, P = 0.0002), REE (259 vs. 26 kcal, P < 0.0001), insulin sensitivity (4 vs. 0.9, P < 0.0001), and β- cell function (7.4 vs. 2.1, P < 0.0001). Conclusions-To our knowledge, this is the first report on the significant advantages of a 6-month hypocaloric HP diet versus hypocaloric HC diet on markers of β- cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in normal, obese females without diabetes.",
author = "Kitabchi, {Abbas E.} and McDaniel, {Kristin A.} and Jim Wan and Frances Tylavsky and Jacovino, {Crystal A.} and Sands, {Chris W.} and Ebenezer Nyenwe and Stentz, {Frankie B.}",
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T1 - Effects of high-protein versus high-carbohydrate diets on markers of β- Cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in obese, premenopausal women without diabetes

AU - Kitabchi, Abbas E.

AU - McDaniel, Kristin A.

AU - Wan, Jim

AU - Tylavsky, Frances

AU - Jacovino, Crystal A.

AU - Sands, Chris W.

AU - Nyenwe, Ebenezer

AU - Stentz, Frankie B.

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N2 - Objective-To study the effects of high-protein versus high-carbohydrate diets on various metabolic end points (glucoregulation, oxidative stress [dichlorofluorescein], lipid peroxidation [malondialdehyde], proinflammatory cytokines [tumor necrosis factor-α and interleukin-6], adipokines, and resting energy expenditure [REE]) with high protein-low carbohydrate (HP) and high carbohydrate-low protein (HC) diets at baseline and after 6 months of dietary intervention. Research design and methods-We recruited obese, premenopausal women aged 20-50 years with no diabetes or prediabetes who were randomized to HC (55% carbohydrates, 30%fat, and 15%protein) or HP (40% carbohydrates, 30%fat, and 30%protein) diets for 6 months. The diets were provided in prepackaged food, which provided 500 kcal restrictions per day. The above metabolic end points were measured with HP and HC diet at baseline and after 6 months of dietary intervention. Results-After 6months of the HP versus HC diet (12 in each group), the following changes were significantly different by Wilcoxon rank sum test for the following parameters: dichloro-fluorescein (-0.8 vs. -0.3 μmol/L, P < 0.0001), malondialdehyDe (-0.4 vs.-0.2 mmol/L, P = 0.0004), C-reactive protein (-2.1 vs.-0.8mg/L, P = 0.0003), E-selectin (-8.6 vs.-3.7 ng/mL, P = 0.0007), adiponectin (1,284 vs. 504 ng/mL, P = 0.0011), tumor necrosis factor-α (-1.8 vs. -0.9 pg/mL, P < 0.0001), IL-6 (-1.3 vs. -0.4 pg/mL, P < 0.0001), free fatty acid (-0.12 vs. 0.16 mmol/L, P = 0.0002), REE (259 vs. 26 kcal, P < 0.0001), insulin sensitivity (4 vs. 0.9, P < 0.0001), and β- cell function (7.4 vs. 2.1, P < 0.0001). Conclusions-To our knowledge, this is the first report on the significant advantages of a 6-month hypocaloric HP diet versus hypocaloric HC diet on markers of β- cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in normal, obese females without diabetes.

AB - Objective-To study the effects of high-protein versus high-carbohydrate diets on various metabolic end points (glucoregulation, oxidative stress [dichlorofluorescein], lipid peroxidation [malondialdehyde], proinflammatory cytokines [tumor necrosis factor-α and interleukin-6], adipokines, and resting energy expenditure [REE]) with high protein-low carbohydrate (HP) and high carbohydrate-low protein (HC) diets at baseline and after 6 months of dietary intervention. Research design and methods-We recruited obese, premenopausal women aged 20-50 years with no diabetes or prediabetes who were randomized to HC (55% carbohydrates, 30%fat, and 15%protein) or HP (40% carbohydrates, 30%fat, and 30%protein) diets for 6 months. The diets were provided in prepackaged food, which provided 500 kcal restrictions per day. The above metabolic end points were measured with HP and HC diet at baseline and after 6 months of dietary intervention. Results-After 6months of the HP versus HC diet (12 in each group), the following changes were significantly different by Wilcoxon rank sum test for the following parameters: dichloro-fluorescein (-0.8 vs. -0.3 μmol/L, P < 0.0001), malondialdehyDe (-0.4 vs.-0.2 mmol/L, P = 0.0004), C-reactive protein (-2.1 vs.-0.8mg/L, P = 0.0003), E-selectin (-8.6 vs.-3.7 ng/mL, P = 0.0007), adiponectin (1,284 vs. 504 ng/mL, P = 0.0011), tumor necrosis factor-α (-1.8 vs. -0.9 pg/mL, P < 0.0001), IL-6 (-1.3 vs. -0.4 pg/mL, P < 0.0001), free fatty acid (-0.12 vs. 0.16 mmol/L, P = 0.0002), REE (259 vs. 26 kcal, P < 0.0001), insulin sensitivity (4 vs. 0.9, P < 0.0001), and β- cell function (7.4 vs. 2.1, P < 0.0001). Conclusions-To our knowledge, this is the first report on the significant advantages of a 6-month hypocaloric HP diet versus hypocaloric HC diet on markers of β- cell function, oxidative stress, lipid peroxidation, proinflammatory cytokines, and adipokines in normal, obese females without diabetes.

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