Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae

Robert P. Lawther, Terrance Cooper

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A number of investigators have shown that 8 to 10 minutes are required before an increase in enzyme activity is observed following addition of various inducers to cultures of Saccharomyces cerevisiae. They have also observed messenger RNA and synthetic capacity half lives of approximately 20 minutes. Our data on the other hand indicate that only 3 minutes elapse between addition of inducer and appearance of active allophanate hydrolase. In addition the capacity of an induced culture to continue producing allophanate hydrolase decays with a half life of 3 minutes following removal of the inducer. These observations raise the possibilities that different species of mRNA possess quite different rates of metabolism or that regulation of the allantoin degradative enzymes may occur at levels other than transcription.

Original languageEnglish (US)
Pages (from-to)1100-1104
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume55
Issue number4
DOIs
StatePublished - Dec 19 1973

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Allophanate Hydrolase
Yeast
Saccharomyces cerevisiae
Allantoin
Messenger RNA
Enzyme activity
Enzymes
Transcription
Metabolism
Half-Life
Research Personnel

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae. / Lawther, Robert P.; Cooper, Terrance.

In: Biochemical and Biophysical Research Communications, Vol. 55, No. 4, 19.12.1973, p. 1100-1104.

Research output: Contribution to journalArticle

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