Effects of interleukin-12 ( IL-12) and endothelial monocyte activated peptide (EMAP-II) treatment on murine pregnancy

R. S. Parhar, K. S. Collison, S. T. Al-Sedairy, David Stern, A. A. Kwaasi, F. A. Al-Mohanna

Research output: Contribution to journalArticle

Abstract

Interleukin IL-12 and EMAP-II have been recently reported lo have anti-tumor and antiangiogenic effects. We tested the hypothesis that the ability of IL-12 to promote TH 1 type response and EMAP-II to inhibit angiogenesis may be effective in controlling pregnancy. The effects of murine IL-12 ( mIL-12) and EMAP-II alone or in combination were examined during the course of pregnancy in CDI mice. Inoculation of mIL-12( 500ng/i.p./12hours/mouse, R&D Systems, USA) between 4.5 and 10 days of pregnancy resulted in a significant fetal resorption. Leukocytes isolated from decidua of resorbing embryos displayed a high degree of adhesion molecule expression and cytotoxicity (p<0.01) towards NK sensitive (YAC-1 cells) as well as NK resistant (auto and allo-trophoblast) cells. Dccidual leukocytes from experimental mice also produced a 10-15 fold higher level of TNF-α and IFN-γ compared to normal pregnani CDI mice . Treatment at day 1.5 to 8 of pregnancy with EMAP-II (2000μg /i.p./mouse/12hours) resulted in either no implantation (>(>60% of mated mice) or embryos with varying degrees of resorption. EMAP-II treatment (500ug) starting on day 5 or later had no significant effect except that the fetuses weighed less than the controls However , litter weights increased by 40-60 % over controls , after a treatment with anti EMAP-II antibody. Treatment with a combination of m IL-12 and EMAP-II (day 4.5 to 10) enhanced the numbers of resorbing embryos . These data suggest thai IL-12 treatment during early pregnancy could result in an activation of decidual leukocytes with potential anti-trophoblast activity leading to fetal demise and that processes involved in reproduction can be controlled by antiangiogenic factors.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

interleukin-12
Interleukin-12
monocytes
Monocytes
pregnancy
peptides
Pregnancy
Peptides
mice
embryo (animal)
Embryonic Structures
leukocytes
Leukocytes
fetal resorption
Fetal Resorption
Decidua
trophoblast
litter weight
Fetal Death
Interleukins

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Parhar, R. S., Collison, K. S., Al-Sedairy, S. T., Stern, D., Kwaasi, A. A., & Al-Mohanna, F. A. (1997). Effects of interleukin-12 ( IL-12) and endothelial monocyte activated peptide (EMAP-II) treatment on murine pregnancy. FASEB Journal, 11(3).

Effects of interleukin-12 ( IL-12) and endothelial monocyte activated peptide (EMAP-II) treatment on murine pregnancy. / Parhar, R. S.; Collison, K. S.; Al-Sedairy, S. T.; Stern, David; Kwaasi, A. A.; Al-Mohanna, F. A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Parhar, RS, Collison, KS, Al-Sedairy, ST, Stern, D, Kwaasi, AA & Al-Mohanna, FA 1997, 'Effects of interleukin-12 ( IL-12) and endothelial monocyte activated peptide (EMAP-II) treatment on murine pregnancy', FASEB Journal, vol. 11, no. 3.
Parhar, R. S. ; Collison, K. S. ; Al-Sedairy, S. T. ; Stern, David ; Kwaasi, A. A. ; Al-Mohanna, F. A. / Effects of interleukin-12 ( IL-12) and endothelial monocyte activated peptide (EMAP-II) treatment on murine pregnancy. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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