Effects of L1 blockade on sensory axon outgrowth and pathfinding in the chick hindlimb

Marcia Honig, Suzanne J. Camilli, Qing Shan Xue

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the developing chick hindlimb, sensory axons, which grow together in bundles as they extend distally, and the motoneuron axons they encounter express the cell adhesion molecule L1. Following injection of function-blocking anti-L1 antibodies into the limb at stage 25, some sensory axons choose inappropriate peripheral nerves even though motoneuron pathfinding is unaffected. Here, to further elucidate L1's role, we assessed the effects of this perturbation using pathway tracing, immune labeling, confocal microscopy, and electron microscopy. After L1 blockade, sensory axons were still bundled and closely apposed. However, clear signs of decreased adhesion were detectable ultrastructurally. Further, sensory axons grew into the limb more slowly than normal, wandering more widely, branching more frequently, and sometimes extending along inappropriate peripheral nerves. Sensory axons that ultimately projected along different cutaneous nerves showed increased intermixing in the spinal nerves, due to errors in pathfinding and also to a decreased ability to segregate into nerve-specific fascicles. These results suggest that, in the highly complex in vivo environment, as in tissue culture, L1 stimulates axon growth and enhances fasciculation, and that these processes contribute to the orderly, timely, and specific growth of sensory axons into the limb.

Original languageEnglish (US)
Pages (from-to)137-154
Number of pages18
JournalDevelopmental Biology
Volume243
Issue number1
DOIs
StatePublished - Mar 1 2002

Fingerprint

Hindlimb
Axons
Extremities
Motor Neurons
Peripheral Nerves
Neural Cell Adhesion Molecule L1
Fasciculation
Spinal Nerves
Blocking Antibodies
Axon Guidance
Neuronal Outgrowth
Growth
Confocal Microscopy
Anti-Idiotypic Antibodies
Electron Microscopy
Skin
Injections

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Effects of L1 blockade on sensory axon outgrowth and pathfinding in the chick hindlimb. / Honig, Marcia; Camilli, Suzanne J.; Xue, Qing Shan.

In: Developmental Biology, Vol. 243, No. 1, 01.03.2002, p. 137-154.

Research output: Contribution to journalArticle

Honig, Marcia ; Camilli, Suzanne J. ; Xue, Qing Shan. / Effects of L1 blockade on sensory axon outgrowth and pathfinding in the chick hindlimb. In: Developmental Biology. 2002 ; Vol. 243, No. 1. pp. 137-154.
@article{50f11bec802a418eba2afaa6acb48629,
title = "Effects of L1 blockade on sensory axon outgrowth and pathfinding in the chick hindlimb",
abstract = "In the developing chick hindlimb, sensory axons, which grow together in bundles as they extend distally, and the motoneuron axons they encounter express the cell adhesion molecule L1. Following injection of function-blocking anti-L1 antibodies into the limb at stage 25, some sensory axons choose inappropriate peripheral nerves even though motoneuron pathfinding is unaffected. Here, to further elucidate L1's role, we assessed the effects of this perturbation using pathway tracing, immune labeling, confocal microscopy, and electron microscopy. After L1 blockade, sensory axons were still bundled and closely apposed. However, clear signs of decreased adhesion were detectable ultrastructurally. Further, sensory axons grew into the limb more slowly than normal, wandering more widely, branching more frequently, and sometimes extending along inappropriate peripheral nerves. Sensory axons that ultimately projected along different cutaneous nerves showed increased intermixing in the spinal nerves, due to errors in pathfinding and also to a decreased ability to segregate into nerve-specific fascicles. These results suggest that, in the highly complex in vivo environment, as in tissue culture, L1 stimulates axon growth and enhances fasciculation, and that these processes contribute to the orderly, timely, and specific growth of sensory axons into the limb.",
author = "Marcia Honig and Camilli, {Suzanne J.} and Xue, {Qing Shan}",
year = "2002",
month = "3",
day = "1",
doi = "10.1006/dbio.2001.0556",
language = "English (US)",
volume = "243",
pages = "137--154",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Effects of L1 blockade on sensory axon outgrowth and pathfinding in the chick hindlimb

AU - Honig, Marcia

AU - Camilli, Suzanne J.

AU - Xue, Qing Shan

PY - 2002/3/1

Y1 - 2002/3/1

N2 - In the developing chick hindlimb, sensory axons, which grow together in bundles as they extend distally, and the motoneuron axons they encounter express the cell adhesion molecule L1. Following injection of function-blocking anti-L1 antibodies into the limb at stage 25, some sensory axons choose inappropriate peripheral nerves even though motoneuron pathfinding is unaffected. Here, to further elucidate L1's role, we assessed the effects of this perturbation using pathway tracing, immune labeling, confocal microscopy, and electron microscopy. After L1 blockade, sensory axons were still bundled and closely apposed. However, clear signs of decreased adhesion were detectable ultrastructurally. Further, sensory axons grew into the limb more slowly than normal, wandering more widely, branching more frequently, and sometimes extending along inappropriate peripheral nerves. Sensory axons that ultimately projected along different cutaneous nerves showed increased intermixing in the spinal nerves, due to errors in pathfinding and also to a decreased ability to segregate into nerve-specific fascicles. These results suggest that, in the highly complex in vivo environment, as in tissue culture, L1 stimulates axon growth and enhances fasciculation, and that these processes contribute to the orderly, timely, and specific growth of sensory axons into the limb.

AB - In the developing chick hindlimb, sensory axons, which grow together in bundles as they extend distally, and the motoneuron axons they encounter express the cell adhesion molecule L1. Following injection of function-blocking anti-L1 antibodies into the limb at stage 25, some sensory axons choose inappropriate peripheral nerves even though motoneuron pathfinding is unaffected. Here, to further elucidate L1's role, we assessed the effects of this perturbation using pathway tracing, immune labeling, confocal microscopy, and electron microscopy. After L1 blockade, sensory axons were still bundled and closely apposed. However, clear signs of decreased adhesion were detectable ultrastructurally. Further, sensory axons grew into the limb more slowly than normal, wandering more widely, branching more frequently, and sometimes extending along inappropriate peripheral nerves. Sensory axons that ultimately projected along different cutaneous nerves showed increased intermixing in the spinal nerves, due to errors in pathfinding and also to a decreased ability to segregate into nerve-specific fascicles. These results suggest that, in the highly complex in vivo environment, as in tissue culture, L1 stimulates axon growth and enhances fasciculation, and that these processes contribute to the orderly, timely, and specific growth of sensory axons into the limb.

UR - http://www.scopus.com/inward/record.url?scp=0036499940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036499940&partnerID=8YFLogxK

U2 - 10.1006/dbio.2001.0556

DO - 10.1006/dbio.2001.0556

M3 - Article

VL - 243

SP - 137

EP - 154

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -