Effects of pre-analytical variables on the anti-activated factor X chromogenic assay when monitoring unfractionated heparin and low molecular weight heparin anticoagulation

David L. McGlasson, Daniel A. Kaczor, Richard A. Krasuski, Charles Campbell, Maria R. Kostur, Joseph T. Adinaro

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The purpose of this study was to determine whether the anti-activated factor X (anti-FXa) assay is less affected by pre-analytical variables in monitoring patients on unfractionated heparin (UFH) and low molecular weight heparin (LMWH) than the activated partial thromboplastin time (aPTT). Forty-six subjects receiving either enoxaparin (LMWH) or UFH were randomly selected. Each study subject had six vacutainer tubes (3.8% sodium citrate, 3.2% sodium citrate) drawn by an atraumatic venipuncture. One tube from each set had a blood to anticoagulant ratio of 9:1. The other tube had an intentional 'short-draw' of approximately 6:1 blood to anticoagulant ratio. All specimens had an aPTT and a chromogenic anti-FXa assay performed on each specimen regardless of heparin type. The aPTT assay mean with the 3.8% sodium citrate tube short-draw tube was statistically different from the other aPTT assays (P = 0.06). However, all six of the mean anti-FXa assays for the UFH and LMWH heparin subjects were not statistically or clinically different (analysis of variance, P = 0.9878 for UFH and P = 0.9060 for LMWH). The intentional short-draw tube did not affect the anti-FXa assay regardless of the anticoagulant. The anti-FXa assay appears to be a better method for monitoring heparin subjects than the aPTT due to the lack of effect of preanalytical variables.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalBlood Coagulation and Fibrinolysis
Volume16
Issue number3
DOIs
StatePublished - Jan 1 2005

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Factor Xa
Low Molecular Weight Heparin
Heparin
Partial Thromboplastin Time
Anticoagulants
Enoxaparin
Phlebotomy
Physiologic Monitoring
Analysis of Variance
sodium citrate

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Effects of pre-analytical variables on the anti-activated factor X chromogenic assay when monitoring unfractionated heparin and low molecular weight heparin anticoagulation. / McGlasson, David L.; Kaczor, Daniel A.; Krasuski, Richard A.; Campbell, Charles; Kostur, Maria R.; Adinaro, Joseph T.

In: Blood Coagulation and Fibrinolysis, Vol. 16, No. 3, 01.01.2005, p. 173-176.

Research output: Contribution to journalArticle

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