Effects of repeated inhalation of toluene on ionotropic GABA A and glutamate receptor subunit levels in rat brain

Jason M. Williams, David Stafford, Jeffery Steketee

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Toluene is a commonly abused solvent found in many industrial and commercial products. The neurobiological effects of toluene remain unclear, but many of them, like those of ethanol, may be mediated by γ-aminobutyric acid (GABA) and glutamate receptors. Chronic ethanol administration has been shown to alter levels of specific subunits for GABA type A (GABA A), N-methyl-d-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors. However, little is known about the effects of toluene on subunit levels of these receptors. To examine this, rats were exposed to toluene vapors (8000 ppm) or air for 10 days (30 min/day), and afterwards GABA A α1, NR1 and NR2B (NMDA) and GluR1 and GluR2/3 (AMPA) receptor subunit levels were determined in discrete brain regions of these animals by Western blotting. Toluene increased GABA A α1, NR1, NR2B and GluR2/3 subunits in the medial prefrontal cortex and decreased GABA A α1 and NR1 subunits in the substantia nigra compacta. Toluene inhalation produced modest increases in GABA A α1 subunits in the striatum, as well as slight decreases in this subunit in the ventral tegmental area. NR2B subunit levels were also slightly increased within the nucleus accumbens by toluene. These studies show that toluene differentially alters the levels of specific GABAergic and glutamatergic receptor subunits in a regionally selective manner.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalNeurochemistry International
Volume46
Issue number1
DOIs
StatePublished - Jan 1 2005

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Glutamate Receptors
Toluene
GABA-A Receptors
Inhalation
gamma-Aminobutyric Acid
Brain
Aspartic Acid
Ethanol
Aminobutyrates
Isoxazoles
Ventral Tegmental Area
GABA Receptors
AMPA Receptors
Nucleus Accumbens
Prefrontal Cortex
Western Blotting
Air

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Effects of repeated inhalation of toluene on ionotropic GABA A and glutamate receptor subunit levels in rat brain. / Williams, Jason M.; Stafford, David; Steketee, Jeffery.

In: Neurochemistry International, Vol. 46, No. 1, 01.01.2005, p. 1-10.

Research output: Contribution to journalArticle

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