Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells

Cha Xiang Guan, Chang Qing Zhang, Xiao Qun Qin, Zi Qiang Luo, Fu Wen Zhou, Xiu Hong Sun

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r = 0. 9703, P <0. 01). (2) VIP (0. 001 ∼ 1 μmol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P <0. 01). (3) Heat stress enhanced the secretion of VIP (P <0. 01) and upregulated the expression of VIPR on BECs (P <0. 05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.

Original languageEnglish (US)
Pages (from-to)103-106
Number of pages4
JournalActa Physiologica Sinica
Volume54
Issue number2
StatePublished - Apr 25 2002

Fingerprint

Vasoactive Intestinal Peptide
Chemotaxis
Epithelial Cells
Vasoactive Intestinal Peptide Receptors
Intestinal Secretions
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Calcium-Calmodulin-Dependent Protein Kinases
Chemotactic Factors
Radio
Protein Kinase C
Radioimmunoassay
Signal Transduction
Epithelium
Hot Temperature
Insulin
Rabbits
Ligands
Lung

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Guan, C. X., Zhang, C. Q., Qin, X. Q., Luo, Z. Q., Zhou, F. W., & Sun, X. H. (2002). Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells. Acta Physiologica Sinica, 54(2), 103-106.

Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells. / Guan, Cha Xiang; Zhang, Chang Qing; Qin, Xiao Qun; Luo, Zi Qiang; Zhou, Fu Wen; Sun, Xiu Hong.

In: Acta Physiologica Sinica, Vol. 54, No. 2, 25.04.2002, p. 103-106.

Research output: Contribution to journalArticle

Guan, CX, Zhang, CQ, Qin, XQ, Luo, ZQ, Zhou, FW & Sun, XH 2002, 'Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells', Acta Physiologica Sinica, vol. 54, no. 2, pp. 103-106.
Guan CX, Zhang CQ, Qin XQ, Luo ZQ, Zhou FW, Sun XH. Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells. Acta Physiologica Sinica. 2002 Apr 25;54(2):103-106.
Guan, Cha Xiang ; Zhang, Chang Qing ; Qin, Xiao Qun ; Luo, Zi Qiang ; Zhou, Fu Wen ; Sun, Xiu Hong. / Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells. In: Acta Physiologica Sinica. 2002 ; Vol. 54, No. 2. pp. 103-106.
@article{5eae2981ab9746cea11bc1aad652f9db,
title = "Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells",
abstract = "To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r = 0. 9703, P <0. 01). (2) VIP (0. 001 ∼ 1 μmol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P <0. 01). (3) Heat stress enhanced the secretion of VIP (P <0. 01) and upregulated the expression of VIPR on BECs (P <0. 05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.",
author = "Guan, {Cha Xiang} and Zhang, {Chang Qing} and Qin, {Xiao Qun} and Luo, {Zi Qiang} and Zhou, {Fu Wen} and Sun, {Xiu Hong}",
year = "2002",
month = "4",
day = "25",
language = "English (US)",
volume = "54",
pages = "103--106",
journal = "Acta Physiologica Sinica",
issn = "0371-0874",
publisher = "Kexue Chubaneshe/Science Press",
number = "2",

}

TY - JOUR

T1 - Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells

AU - Guan, Cha Xiang

AU - Zhang, Chang Qing

AU - Qin, Xiao Qun

AU - Luo, Zi Qiang

AU - Zhou, Fu Wen

AU - Sun, Xiu Hong

PY - 2002/4/25

Y1 - 2002/4/25

N2 - To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r = 0. 9703, P <0. 01). (2) VIP (0. 001 ∼ 1 μmol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P <0. 01). (3) Heat stress enhanced the secretion of VIP (P <0. 01) and upregulated the expression of VIPR on BECs (P <0. 05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.

AB - To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r = 0. 9703, P <0. 01). (2) VIP (0. 001 ∼ 1 μmol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P <0. 01). (3) Heat stress enhanced the secretion of VIP (P <0. 01) and upregulated the expression of VIPR on BECs (P <0. 05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.

UR - http://www.scopus.com/inward/record.url?scp=1842854233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842854233&partnerID=8YFLogxK

M3 - Article

VL - 54

SP - 103

EP - 106

JO - Acta Physiologica Sinica

JF - Acta Physiologica Sinica

SN - 0371-0874

IS - 2

ER -