Effects on gene expressions of G protein alpha subunits by activation of non-neuronal muscarinic receptor

Kai Chen, Hai Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: To study the subtypes distribution of G-proteins and modulation by carbachol and pilocarpine on gene expression in endothelial cells. Methods: The rat aorta endothelial cells were cultivated and incubated with carbachol and pilocarpine at the dose of 10-4 mol · L-1 for 6 h. Then the total RNA was extracted. The mRNA levels of G-protein α subunits was measured by RT-PCR. Results: Gq/11, Gs and Gi mRNA was detected in rat aorta endothelial cells, while G12/13 mRNA was not detected. Carbachol and pilocarpine treatment induced no changes in Gs, Gi and G11 mRNA. Gq mRNA was 72.7% up-regulated by carbachol and unchanged by pilocarpine. Conclusion: In all G-protein α subunits, only Gq mRNA was changed after activation of non-neuronal muscarinic receptor by carbachol. We can conclude that Gq-protein may play an important role in signal transduction of non-neuronal muscarinic receptor.

Original languageEnglish (US)
Pages (from-to)664-667
Number of pages4
JournalChinese Pharmacological Bulletin
Volume22
Issue number6
StatePublished - Jan 1 2006
Externally publishedYes

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GTP-Binding Protein alpha Subunits
Muscarinic Receptors
Carbachol
Pilocarpine
Gene Expression
Messenger RNA
GTP-Binding Proteins
Endothelial Cells
Protein Subunits
Aorta
Gq-G11 GTP-Binding Protein alpha Subunits
Signal Transduction
RNA
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Effects on gene expressions of G protein alpha subunits by activation of non-neuronal muscarinic receptor. / Chen, Kai; Wang, Hai.

In: Chinese Pharmacological Bulletin, Vol. 22, No. 6, 01.01.2006, p. 664-667.

Research output: Contribution to journalArticle

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abstract = "Aim: To study the subtypes distribution of G-proteins and modulation by carbachol and pilocarpine on gene expression in endothelial cells. Methods: The rat aorta endothelial cells were cultivated and incubated with carbachol and pilocarpine at the dose of 10-4 mol · L-1 for 6 h. Then the total RNA was extracted. The mRNA levels of G-protein α subunits was measured by RT-PCR. Results: Gq/11, Gs and Gi mRNA was detected in rat aorta endothelial cells, while G12/13 mRNA was not detected. Carbachol and pilocarpine treatment induced no changes in Gs, Gi and G11 mRNA. Gq mRNA was 72.7{\%} up-regulated by carbachol and unchanged by pilocarpine. Conclusion: In all G-protein α subunits, only Gq mRNA was changed after activation of non-neuronal muscarinic receptor by carbachol. We can conclude that Gq-protein may play an important role in signal transduction of non-neuronal muscarinic receptor.",
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AB - Aim: To study the subtypes distribution of G-proteins and modulation by carbachol and pilocarpine on gene expression in endothelial cells. Methods: The rat aorta endothelial cells were cultivated and incubated with carbachol and pilocarpine at the dose of 10-4 mol · L-1 for 6 h. Then the total RNA was extracted. The mRNA levels of G-protein α subunits was measured by RT-PCR. Results: Gq/11, Gs and Gi mRNA was detected in rat aorta endothelial cells, while G12/13 mRNA was not detected. Carbachol and pilocarpine treatment induced no changes in Gs, Gi and G11 mRNA. Gq mRNA was 72.7% up-regulated by carbachol and unchanged by pilocarpine. Conclusion: In all G-protein α subunits, only Gq mRNA was changed after activation of non-neuronal muscarinic receptor by carbachol. We can conclude that Gq-protein may play an important role in signal transduction of non-neuronal muscarinic receptor.

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