Efficiency of nanotube surface-treated dental implants loaded with doxycycline on growth reduction of Porphyromonas gingivalis

Cimara Ferreira, Jegdish Babu, Azhang Hamlekhan, Sweetu Patel, Tolou Shokuhfar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: The prevalence of peri-implant infection in patients with dental implants has been shown to range from 28% to 56%. A nanotube-modified implant surface can deliver antibiotics locally and suppress periodontal pathogenic bacterial growth. The aim of this study was to evaluate the deliverability of antibiotics via a nanotube-modified implant. Materials and Methods: Dental implants with a nanotube surface were fabricated and loaded with doxycycline. Afterward, each dental implant with a nanotube surface was placed into 2-mL tubes, removed from solution, and placed in a fresh solution daily for 28 days. Experimental samples from 1, 2, 4, 16, 24, and 28 days were used for this evaluation. The concentration of doxycycline was measured using spectrophotometric analysis at 273-nm absorbance. The antibacterial effect of doxycycline was evaluated by supplementing Porphyromonas gingivalis (P gingivalis) growth media with the solution collected from the dental implants at the aforementioned time intervals for a period of 48 hours under anaerobic conditions. A bacterial viability assay was used to evaluate P gingivalis growth at 550-nm absorbance. Results: Doxycycline concentration varied from 0.33 to 1.22 μg/mL from day 1 to day 28, respectively. A bacterial viability assay showed the highest P gingivalis growth at day 1 (2 nm) and the lowest at day 4 (0.17 nm), with a gradual reduction from day 1 to day 4 of approximately 87.5%. The subsequent growth pattern was maintained and slightly increased from baseline in approximately 48.3% from day 1 to day 24. The final P gingivalis growth measured at day 28 was 29.4% less than the baseline growth. Conclusion: P gingivalis growth was suppressed in media supplemented with solution collected from dental implants with a nanotube surface loaded with doxycycline during a 28-day time interval.

Original languageEnglish (US)
Pages (from-to)322-328
Number of pages7
JournalInternational Journal of Oral and Maxillofacial Implants
Volume32
Issue number2
DOIs
StatePublished - Jan 1 2017

Fingerprint

Nanotubes
Porphyromonas gingivalis
Dental Implants
Doxycycline
Growth
Microbial Viability
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Efficiency of nanotube surface-treated dental implants loaded with doxycycline on growth reduction of Porphyromonas gingivalis. / Ferreira, Cimara; Babu, Jegdish; Hamlekhan, Azhang; Patel, Sweetu; Shokuhfar, Tolou.

In: International Journal of Oral and Maxillofacial Implants, Vol. 32, No. 2, 01.01.2017, p. 322-328.

Research output: Contribution to journalArticle

@article{83106449bd3445f68e824ac27d0ee6a8,
title = "Efficiency of nanotube surface-treated dental implants loaded with doxycycline on growth reduction of Porphyromonas gingivalis",
abstract = "Purpose: The prevalence of peri-implant infection in patients with dental implants has been shown to range from 28{\%} to 56{\%}. A nanotube-modified implant surface can deliver antibiotics locally and suppress periodontal pathogenic bacterial growth. The aim of this study was to evaluate the deliverability of antibiotics via a nanotube-modified implant. Materials and Methods: Dental implants with a nanotube surface were fabricated and loaded with doxycycline. Afterward, each dental implant with a nanotube surface was placed into 2-mL tubes, removed from solution, and placed in a fresh solution daily for 28 days. Experimental samples from 1, 2, 4, 16, 24, and 28 days were used for this evaluation. The concentration of doxycycline was measured using spectrophotometric analysis at 273-nm absorbance. The antibacterial effect of doxycycline was evaluated by supplementing Porphyromonas gingivalis (P gingivalis) growth media with the solution collected from the dental implants at the aforementioned time intervals for a period of 48 hours under anaerobic conditions. A bacterial viability assay was used to evaluate P gingivalis growth at 550-nm absorbance. Results: Doxycycline concentration varied from 0.33 to 1.22 μg/mL from day 1 to day 28, respectively. A bacterial viability assay showed the highest P gingivalis growth at day 1 (2 nm) and the lowest at day 4 (0.17 nm), with a gradual reduction from day 1 to day 4 of approximately 87.5{\%}. The subsequent growth pattern was maintained and slightly increased from baseline in approximately 48.3{\%} from day 1 to day 24. The final P gingivalis growth measured at day 28 was 29.4{\%} less than the baseline growth. Conclusion: P gingivalis growth was suppressed in media supplemented with solution collected from dental implants with a nanotube surface loaded with doxycycline during a 28-day time interval.",
author = "Cimara Ferreira and Jegdish Babu and Azhang Hamlekhan and Sweetu Patel and Tolou Shokuhfar",
year = "2017",
month = "1",
day = "1",
doi = "10.11607/jomi.4975",
language = "English (US)",
volume = "32",
pages = "322--328",
journal = "International Journal of Oral and Maxillofacial Implants",
issn = "0882-2786",
publisher = "Quintessence Publishing Company",
number = "2",

}

TY - JOUR

T1 - Efficiency of nanotube surface-treated dental implants loaded with doxycycline on growth reduction of Porphyromonas gingivalis

AU - Ferreira, Cimara

AU - Babu, Jegdish

AU - Hamlekhan, Azhang

AU - Patel, Sweetu

AU - Shokuhfar, Tolou

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Purpose: The prevalence of peri-implant infection in patients with dental implants has been shown to range from 28% to 56%. A nanotube-modified implant surface can deliver antibiotics locally and suppress periodontal pathogenic bacterial growth. The aim of this study was to evaluate the deliverability of antibiotics via a nanotube-modified implant. Materials and Methods: Dental implants with a nanotube surface were fabricated and loaded with doxycycline. Afterward, each dental implant with a nanotube surface was placed into 2-mL tubes, removed from solution, and placed in a fresh solution daily for 28 days. Experimental samples from 1, 2, 4, 16, 24, and 28 days were used for this evaluation. The concentration of doxycycline was measured using spectrophotometric analysis at 273-nm absorbance. The antibacterial effect of doxycycline was evaluated by supplementing Porphyromonas gingivalis (P gingivalis) growth media with the solution collected from the dental implants at the aforementioned time intervals for a period of 48 hours under anaerobic conditions. A bacterial viability assay was used to evaluate P gingivalis growth at 550-nm absorbance. Results: Doxycycline concentration varied from 0.33 to 1.22 μg/mL from day 1 to day 28, respectively. A bacterial viability assay showed the highest P gingivalis growth at day 1 (2 nm) and the lowest at day 4 (0.17 nm), with a gradual reduction from day 1 to day 4 of approximately 87.5%. The subsequent growth pattern was maintained and slightly increased from baseline in approximately 48.3% from day 1 to day 24. The final P gingivalis growth measured at day 28 was 29.4% less than the baseline growth. Conclusion: P gingivalis growth was suppressed in media supplemented with solution collected from dental implants with a nanotube surface loaded with doxycycline during a 28-day time interval.

AB - Purpose: The prevalence of peri-implant infection in patients with dental implants has been shown to range from 28% to 56%. A nanotube-modified implant surface can deliver antibiotics locally and suppress periodontal pathogenic bacterial growth. The aim of this study was to evaluate the deliverability of antibiotics via a nanotube-modified implant. Materials and Methods: Dental implants with a nanotube surface were fabricated and loaded with doxycycline. Afterward, each dental implant with a nanotube surface was placed into 2-mL tubes, removed from solution, and placed in a fresh solution daily for 28 days. Experimental samples from 1, 2, 4, 16, 24, and 28 days were used for this evaluation. The concentration of doxycycline was measured using spectrophotometric analysis at 273-nm absorbance. The antibacterial effect of doxycycline was evaluated by supplementing Porphyromonas gingivalis (P gingivalis) growth media with the solution collected from the dental implants at the aforementioned time intervals for a period of 48 hours under anaerobic conditions. A bacterial viability assay was used to evaluate P gingivalis growth at 550-nm absorbance. Results: Doxycycline concentration varied from 0.33 to 1.22 μg/mL from day 1 to day 28, respectively. A bacterial viability assay showed the highest P gingivalis growth at day 1 (2 nm) and the lowest at day 4 (0.17 nm), with a gradual reduction from day 1 to day 4 of approximately 87.5%. The subsequent growth pattern was maintained and slightly increased from baseline in approximately 48.3% from day 1 to day 24. The final P gingivalis growth measured at day 28 was 29.4% less than the baseline growth. Conclusion: P gingivalis growth was suppressed in media supplemented with solution collected from dental implants with a nanotube surface loaded with doxycycline during a 28-day time interval.

UR - http://www.scopus.com/inward/record.url?scp=85015735028&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015735028&partnerID=8YFLogxK

U2 - 10.11607/jomi.4975

DO - 10.11607/jomi.4975

M3 - Article

VL - 32

SP - 322

EP - 328

JO - International Journal of Oral and Maxillofacial Implants

JF - International Journal of Oral and Maxillofacial Implants

SN - 0882-2786

IS - 2

ER -