Efficient identification of recombinant adenoviruses by direct plaque screening

Yi Lu, Yu Zhang, Mitchell S. Steiner

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

With the increasing use of adenoviral vectors for gene transfer and gene therapy, it is crucial to produce specific recombinant adenoviruses more efficiently. One of the most time-consuming steps is to screen the unique recombinant adenovirus among the plaques, in which each plaque has to be amplified individually in kidney 293 cells in order to obtain enough adenoviruses for DNA extraction and subsequent identification. We have developed a fast and simple way to screen recombinant adenoviruses by direct plaque screening. The direct plaque-screening method employed DNA obtained from the viral plaque itself for PCR amplification and subsequent adenoviral recombinant identification. The time and labor involved in these steps has been significantly reduced.

Original languageEnglish (US)
Pages (from-to)643-645
Number of pages3
JournalDNA and Cell Biology
Volume17
Issue number7
DOIs
StatePublished - Jan 1 1998

Fingerprint

Adenoviridae
Viral DNA
Genetic Therapy
Kidney
Polymerase Chain Reaction
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Efficient identification of recombinant adenoviruses by direct plaque screening. / Lu, Yi; Zhang, Yu; Steiner, Mitchell S.

In: DNA and Cell Biology, Vol. 17, No. 7, 01.01.1998, p. 643-645.

Research output: Contribution to journalArticle

Lu, Yi ; Zhang, Yu ; Steiner, Mitchell S. / Efficient identification of recombinant adenoviruses by direct plaque screening. In: DNA and Cell Biology. 1998 ; Vol. 17, No. 7. pp. 643-645.
@article{8e186a965b834ac2853dd69ae6721e48,
title = "Efficient identification of recombinant adenoviruses by direct plaque screening",
abstract = "With the increasing use of adenoviral vectors for gene transfer and gene therapy, it is crucial to produce specific recombinant adenoviruses more efficiently. One of the most time-consuming steps is to screen the unique recombinant adenovirus among the plaques, in which each plaque has to be amplified individually in kidney 293 cells in order to obtain enough adenoviruses for DNA extraction and subsequent identification. We have developed a fast and simple way to screen recombinant adenoviruses by direct plaque screening. The direct plaque-screening method employed DNA obtained from the viral plaque itself for PCR amplification and subsequent adenoviral recombinant identification. The time and labor involved in these steps has been significantly reduced.",
author = "Yi Lu and Yu Zhang and Steiner, {Mitchell S.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1089/dna.1998.17.643",
language = "English (US)",
volume = "17",
pages = "643--645",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "7",

}

TY - JOUR

T1 - Efficient identification of recombinant adenoviruses by direct plaque screening

AU - Lu, Yi

AU - Zhang, Yu

AU - Steiner, Mitchell S.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - With the increasing use of adenoviral vectors for gene transfer and gene therapy, it is crucial to produce specific recombinant adenoviruses more efficiently. One of the most time-consuming steps is to screen the unique recombinant adenovirus among the plaques, in which each plaque has to be amplified individually in kidney 293 cells in order to obtain enough adenoviruses for DNA extraction and subsequent identification. We have developed a fast and simple way to screen recombinant adenoviruses by direct plaque screening. The direct plaque-screening method employed DNA obtained from the viral plaque itself for PCR amplification and subsequent adenoviral recombinant identification. The time and labor involved in these steps has been significantly reduced.

AB - With the increasing use of adenoviral vectors for gene transfer and gene therapy, it is crucial to produce specific recombinant adenoviruses more efficiently. One of the most time-consuming steps is to screen the unique recombinant adenovirus among the plaques, in which each plaque has to be amplified individually in kidney 293 cells in order to obtain enough adenoviruses for DNA extraction and subsequent identification. We have developed a fast and simple way to screen recombinant adenoviruses by direct plaque screening. The direct plaque-screening method employed DNA obtained from the viral plaque itself for PCR amplification and subsequent adenoviral recombinant identification. The time and labor involved in these steps has been significantly reduced.

UR - http://www.scopus.com/inward/record.url?scp=0031816463&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031816463&partnerID=8YFLogxK

U2 - 10.1089/dna.1998.17.643

DO - 10.1089/dna.1998.17.643

M3 - Article

VL - 17

SP - 643

EP - 645

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 7

ER -