Efficient PCR-based random mutagenesis of sub-genic (100 bp) DNA fragments

Vladimir Svetlov, Terrance Cooper

Research output: Contribution to journalShort survey

14 Citations (Scopus)

Abstract

Here we describe a method for performing a PCR-driven random mutagenesis of 100 bp DNA fragments that yields mutations at a useful frequency. The method is a modification of the manganese ion substitution PCR technique, and neither creates 'hot-spots' nor favors transition mutations over transversions.

Original languageEnglish (US)
Pages (from-to)89-91
Number of pages3
JournalYeast
Volume14
Issue number1
DOIs
StatePublished - Jan 15 1998

Fingerprint

Mutagenesis
Manganese
mutagenesis
DNA
Substitution reactions
Ions
Polymerase Chain Reaction
Mutation
mutation
manganese
methodology
ions

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Efficient PCR-based random mutagenesis of sub-genic (100 bp) DNA fragments. / Svetlov, Vladimir; Cooper, Terrance.

In: Yeast, Vol. 14, No. 1, 15.01.1998, p. 89-91.

Research output: Contribution to journalShort survey

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