Eicosanoid synthesis elicited by norepinephrine in piglet parietal cortex

David W. Busija, Charles Leffler

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We investigated effects of exogenous norepinephrine and isoproterenol on pial arterial diameter and cerebral eicosanoid synthesis in anesthetized newborn pigs. Norepinephrine in artificial cerebrospinal fluid (CSF) constricted pial arteries from 203 ± 27 μm (X ± S.E.M.) to 164 ± 18 μm (20 ± 2%) (n = 21 vessels from 16 animals) at 10-4 M. In the same animals, norepinephrine caused the concentration in CSF of 6-keto-prostaglandin F to increase from 768 ± 91 to 1544 ± 151 pg/ml, thromboxane B2 to increase from 188 ± 37 to 269 ± 38 pg/ml, and prostaglandin E2 to increase from 2067 ± 448 to 6575 ± 751 pg/ml. Topical application of prostaglandin E2 in CSF to the cortical surface demonstrated that concentrations as low as 10,000 pg/ml were able to dilate pial arteries substantially. Blockade of cyclo-oxygenase activity by indomethacin (5-10 mg/kg, i.v.) potentiated pial arterial constriction to norepinephrine. Topical isoproterenol dilated pial arteries, but isoproterenol did not affect levels of measured eicosanoids in CSF. We conclude that norepinephrine elicits release of prostanoids from the cortical surface, and that these substances limit cerebrovascular constriction to norepinephrine.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalBrain Research
Volume403
Issue number2
DOIs
StatePublished - Feb 17 1987

Fingerprint

Parietal Lobe
Eicosanoids
Norepinephrine
Cerebrospinal Fluid
Isoproterenol
Arteries
Dinoprostone
Constriction
Thromboxane B2
Prostaglandin-Endoperoxide Synthases
Indomethacin
Prostaglandins
Swine

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

Eicosanoid synthesis elicited by norepinephrine in piglet parietal cortex. / Busija, David W.; Leffler, Charles.

In: Brain Research, Vol. 403, No. 2, 17.02.1987, p. 243-248.

Research output: Contribution to journalArticle

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