Elevated oxidative stress and sensorimotor deficits but normal cognition in mice that cannot synthesize ascorbic acid

Fiona E. Harrison, Sarah S. Yu, Kristen L. Van Den Bossche, Liying Li, James M. May, Michael Mcdonald

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Oxidative stress is implicated in the cognitive deterioration associated with normal aging as well as neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. We investigated the effect of ascorbic acid (vitamin C) on oxidative stress, cognition, and motor abilities in mice null for gulono-γ-lactone oxidase (Gulo). Gulo-/- mice are unable to synthesize ascorbic acid and depend on dietary ascorbic acid for survival. Gulo-/- mice were given supplements that provided them either with ascorbic acid levels equal to- or slightly higher than wild-type mice (Gulo-sufficient), or lower than physiological levels (Gulo-low) that were just enough to prevent scurvy. Ascorbic acid is a major anti-oxidant in mice and any reduction in ascorbic acid level is therefore likely to result in increased oxidative stress. Ascorbic acid levels in the brain and liver were higher in Gulo-sufficient mice than in Gulo-low mice. F4-neuroprostanes were elevated in cortex and cerebellum in Gulo-low mice and in the cortex of Gulo-sufficient mice. All Gulo-/- mice were cognitively normal but had a strength and agility deficit that was worse in Gulo-low mice. This suggests that low levels of ascorbic acid and elevated oxidative stress as measured by F4-neuroprostanes alone are insufficient to impair memory in the knockouts but may be responsible for the exacerbated motor deficits in Gulo-low mice, and ascorbic acid may have a vital role in maintaining motor abilities.

Original languageEnglish (US)
Pages (from-to)1198-1208
Number of pages11
JournalJournal of Neurochemistry
Volume106
Issue number3
DOIs
StatePublished - Aug 1 2008

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Oxidative stress
Lactones
Cognition
Ascorbic Acid
Oxidoreductases
Oxidative Stress
Neuroprostanes
Aptitude
Scurvy
Oxidants
Neurodegenerative Diseases
Liver
Cerebellum
Parkinson Disease
Deterioration
Brain
Alzheimer Disease
Aging of materials

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Elevated oxidative stress and sensorimotor deficits but normal cognition in mice that cannot synthesize ascorbic acid. / Harrison, Fiona E.; Yu, Sarah S.; Van Den Bossche, Kristen L.; Li, Liying; May, James M.; Mcdonald, Michael.

In: Journal of Neurochemistry, Vol. 106, No. 3, 01.08.2008, p. 1198-1208.

Research output: Contribution to journalArticle

Harrison, Fiona E. ; Yu, Sarah S. ; Van Den Bossche, Kristen L. ; Li, Liying ; May, James M. ; Mcdonald, Michael. / Elevated oxidative stress and sensorimotor deficits but normal cognition in mice that cannot synthesize ascorbic acid. In: Journal of Neurochemistry. 2008 ; Vol. 106, No. 3. pp. 1198-1208.
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