Elevation of intracellular Ca2+ concentration in rabbit nonpigmented ciliary epithelial cells by allicin

Teh Ching Chu, Jarrett L. Burch, Marco A. De Paula Brotto, Tony L. Creazzo, Joan Han, Grace Y. Han, David E. Potter

Research output: Contribution to journalArticle

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Abstract

A previous study has shown that allicin produces changes in aqueous humor dynamics, and this study was conducted to examine possible cellular mechanisms. In rabbit nonpigmented ciliary epithelial cells, basal levels of [Ca2+](i) were determined to be 164 ± 34 nM. Allicin, a sulfhydryl-reactive agent, induced Ca2+ transients at 0.01 mM and at 0.2 mM, the Ca2+ transient peaked at 732 ± 33 nM. Allicin-induced Ca2+ transients were prevented by pretreatment with dithiothreitol which did not affect the basal Ca2+ levels. Allicin had only a slight, insignificant, effect on L-type Ca2+ currents, and allicin-induced Ca2+ transients were also present under extracellular Ca2+-free conditions. These data suggest that intracellular Ca2+ stores are the most probable source of allicin's effect. Pretreatment of cells with ryanodine, an inhibitor of Ca2+-induced-Ca2+-release, inhibited allicin-induced Ca2+ transients, but the basal Ca2+ levels were unaffected by ryanodine. Thus, allicin-induced Ca2+ transients are most likely mediated through ryanodine-sensitive intracellular Ca2+ stores. Copyright (C) 1996 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume115
Issue number1
DOIs
StatePublished - Sep 1 1996

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Epithelial Cells
Rabbits
Ryanodine
allicin
Aqueous Humor
Dithiothreitol

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

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Elevation of intracellular Ca2+ concentration in rabbit nonpigmented ciliary epithelial cells by allicin. / Chu, Teh Ching; Burch, Jarrett L.; De Paula Brotto, Marco A.; Creazzo, Tony L.; Han, Joan; Han, Grace Y.; Potter, David E.

In: Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology, Vol. 115, No. 1, 01.09.1996, p. 89-94.

Research output: Contribution to journalArticle

Chu, Teh Ching ; Burch, Jarrett L. ; De Paula Brotto, Marco A. ; Creazzo, Tony L. ; Han, Joan ; Han, Grace Y. ; Potter, David E. / Elevation of intracellular Ca2+ concentration in rabbit nonpigmented ciliary epithelial cells by allicin. In: Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology. 1996 ; Vol. 115, No. 1. pp. 89-94.
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