Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity

B. Brocato, A. A. Zoerner, Z. Janjetovic, C. Skobowiat, S. Gupta, Bob Moore, A. Slominski, Jie Zhang, Mauro Schenone, R. Phinehas, R. J. Ferry, E. Dick, G. B. Hubbard, Giancarlo Mari, N. Schlabritz-Loutsevitch

Research output: Contribution to journalArticle

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Abstract

Introduction Maternal obesity (MO) remains a serious obstetric problem with acute and chronic morbidities for both mothers and offspring. The mechanisms underlying these adverse consequences of MO remain unknown. Endocannabinoids (ECB) are neuromodulatory lipids released from adipocytes and other tissues. Metabolic crosstalk between placenta and adipocytes may mediate sequelae of MO. The goal of this study was to elucidate placental and systemic ECB in MO. Material and methods Placentas, sera, and subcutaneous fat were collected at Cesarean sections performed near term (0.9 G) in four non-obese (nOB) and four obese (OB) baboons (Papio spp.). Concentrations of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were measured by liquid chromatography coupled to tandem mass spectrometry. AEA and 2-AG pathways were characterized in placentas by Q-RT-PCR, Western blot and immunohistochemistry. Results Placental 2-AG levels were lower and maternal fat AEA levels were higher in OB (1254.1 ± 401.3 nmol/kg and 17.3 ± 4 nmol/kg) vs. nOB (3124.2 ± 557.3 nmol/kg and 3.1 ± 0.6 nmol/kg) animals. Concentrations of 2-AG correlated positively between maternal fat and placenta (r = 0.82, p = 0.013), but correlated negatively with maternal leptin concentrations (r = -0.72, p = 0.04 and r = -0.83, p = 0.01, respectively). Conclusion This is the first study to demonstrate differential ECB pathway regulation in maternal fat and placenta in MO. Differential regulation and function exist for AEA and 2-AG as the major ECB pathways in placenta.

Original languageEnglish (US)
Pages (from-to)983-989
Number of pages7
JournalPlacenta
Volume34
Issue number11
DOIs
StatePublished - Nov 1 2013

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Endocannabinoids
Papio
Placenta
Obesity
Fats
Mothers
Adipocytes
Subcutaneous Fat
Tandem Mass Spectrometry
Leptin
Liquid Chromatography
Cesarean Section
Obstetrics
Western Blotting
Immunohistochemistry
2-arachidonylglycerol
Morbidity
Lipids
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Brocato, B., Zoerner, A. A., Janjetovic, Z., Skobowiat, C., Gupta, S., Moore, B., ... Schlabritz-Loutsevitch, N. (2013). Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity. Placenta, 34(11), 983-989. https://doi.org/10.1016/j.placenta.2013.08.007

Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity. / Brocato, B.; Zoerner, A. A.; Janjetovic, Z.; Skobowiat, C.; Gupta, S.; Moore, Bob; Slominski, A.; Zhang, Jie; Schenone, Mauro; Phinehas, R.; Ferry, R. J.; Dick, E.; Hubbard, G. B.; Mari, Giancarlo; Schlabritz-Loutsevitch, N.

In: Placenta, Vol. 34, No. 11, 01.11.2013, p. 983-989.

Research output: Contribution to journalArticle

Brocato, B, Zoerner, AA, Janjetovic, Z, Skobowiat, C, Gupta, S, Moore, B, Slominski, A, Zhang, J, Schenone, M, Phinehas, R, Ferry, RJ, Dick, E, Hubbard, GB, Mari, G & Schlabritz-Loutsevitch, N 2013, 'Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity', Placenta, vol. 34, no. 11, pp. 983-989. https://doi.org/10.1016/j.placenta.2013.08.007
Brocato, B. ; Zoerner, A. A. ; Janjetovic, Z. ; Skobowiat, C. ; Gupta, S. ; Moore, Bob ; Slominski, A. ; Zhang, Jie ; Schenone, Mauro ; Phinehas, R. ; Ferry, R. J. ; Dick, E. ; Hubbard, G. B. ; Mari, Giancarlo ; Schlabritz-Loutsevitch, N. / Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity. In: Placenta. 2013 ; Vol. 34, No. 11. pp. 983-989.
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T1 - Endocannabinoid crosstalk between placenta and maternal fat in a baboon model (Papio spp.) of obesity

AU - Brocato, B.

AU - Zoerner, A. A.

AU - Janjetovic, Z.

AU - Skobowiat, C.

AU - Gupta, S.

AU - Moore, Bob

AU - Slominski, A.

AU - Zhang, Jie

AU - Schenone, Mauro

AU - Phinehas, R.

AU - Ferry, R. J.

AU - Dick, E.

AU - Hubbard, G. B.

AU - Mari, Giancarlo

AU - Schlabritz-Loutsevitch, N.

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Introduction Maternal obesity (MO) remains a serious obstetric problem with acute and chronic morbidities for both mothers and offspring. The mechanisms underlying these adverse consequences of MO remain unknown. Endocannabinoids (ECB) are neuromodulatory lipids released from adipocytes and other tissues. Metabolic crosstalk between placenta and adipocytes may mediate sequelae of MO. The goal of this study was to elucidate placental and systemic ECB in MO. Material and methods Placentas, sera, and subcutaneous fat were collected at Cesarean sections performed near term (0.9 G) in four non-obese (nOB) and four obese (OB) baboons (Papio spp.). Concentrations of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were measured by liquid chromatography coupled to tandem mass spectrometry. AEA and 2-AG pathways were characterized in placentas by Q-RT-PCR, Western blot and immunohistochemistry. Results Placental 2-AG levels were lower and maternal fat AEA levels were higher in OB (1254.1 ± 401.3 nmol/kg and 17.3 ± 4 nmol/kg) vs. nOB (3124.2 ± 557.3 nmol/kg and 3.1 ± 0.6 nmol/kg) animals. Concentrations of 2-AG correlated positively between maternal fat and placenta (r = 0.82, p = 0.013), but correlated negatively with maternal leptin concentrations (r = -0.72, p = 0.04 and r = -0.83, p = 0.01, respectively). Conclusion This is the first study to demonstrate differential ECB pathway regulation in maternal fat and placenta in MO. Differential regulation and function exist for AEA and 2-AG as the major ECB pathways in placenta.

AB - Introduction Maternal obesity (MO) remains a serious obstetric problem with acute and chronic morbidities for both mothers and offspring. The mechanisms underlying these adverse consequences of MO remain unknown. Endocannabinoids (ECB) are neuromodulatory lipids released from adipocytes and other tissues. Metabolic crosstalk between placenta and adipocytes may mediate sequelae of MO. The goal of this study was to elucidate placental and systemic ECB in MO. Material and methods Placentas, sera, and subcutaneous fat were collected at Cesarean sections performed near term (0.9 G) in four non-obese (nOB) and four obese (OB) baboons (Papio spp.). Concentrations of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were measured by liquid chromatography coupled to tandem mass spectrometry. AEA and 2-AG pathways were characterized in placentas by Q-RT-PCR, Western blot and immunohistochemistry. Results Placental 2-AG levels were lower and maternal fat AEA levels were higher in OB (1254.1 ± 401.3 nmol/kg and 17.3 ± 4 nmol/kg) vs. nOB (3124.2 ± 557.3 nmol/kg and 3.1 ± 0.6 nmol/kg) animals. Concentrations of 2-AG correlated positively between maternal fat and placenta (r = 0.82, p = 0.013), but correlated negatively with maternal leptin concentrations (r = -0.72, p = 0.04 and r = -0.83, p = 0.01, respectively). Conclusion This is the first study to demonstrate differential ECB pathway regulation in maternal fat and placenta in MO. Differential regulation and function exist for AEA and 2-AG as the major ECB pathways in placenta.

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