Endogenous surface phosphorylation reactions and ectokinase activity in the guinea pig T lymphocyte

Ellen S. Kang, Arnold Postlethwaite, Sandeford Schaeffer, Baljeet Sawhney

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The guinea pig T lymphocyte, known to interact with other cells via direct cell-to-cell contact, exhibits endogenous surface kinase activity as reflected by the appearance of four major labeled bands in autoradiographs of dried sodium dodecyl sulfate (NaDoSO4)-polyacrylamide gel electrophoresis gels when intact cells are briefly exposed to micromolar concentrations of [γ-32P]ATP followed immediately by solubilization with NaDoSO4 to terminate the reaction. This pattern differs from the labeling of intracellular components which is seen when intact cells are incubated with 32PO4 to generate intracellular [γ-32P]ATP when only two major labeled bands of protein with different molecular weights are seen. Of a number of modulators of lymphocyte function tested, cyclic GMP and phytohemagglutinin (PHA) caused additional bands to appear in cells exposed to [γ-32P]ATP. The labeling of added casein was catalyzed by intact cells harvested 4 weeks after injection of animals with Freund's complete adjuvant but not earlier. These findings indicate that plasma membrane kinase activity of guinea pig T lymphocytes is accessible to the extracellular environs (ectokinase activity) and to endogenous surface substrates and that the limitation for such reactions is the availability of ATP in the extracellular component. In view of the number of circumstances under which ATP could appear outside of cells for brief periods of time, these reactions could well take place in vivo.

Original languageEnglish (US)
Pages (from-to)319-326
Number of pages8
JournalCellular Immunology
Volume87
Issue number2
DOIs
StatePublished - Jan 1 1984

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Guinea Pigs
Phosphorylation
T-Lymphocytes
Adenosine Triphosphate
Phosphotransferases
Freund's Adjuvant
ectoprotein kinase
Cyclic GMP
Lymphocyte Count
Phytohemagglutinins
Caseins
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Molecular Weight
Gels
Cell Membrane
Injections
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Endogenous surface phosphorylation reactions and ectokinase activity in the guinea pig T lymphocyte. / Kang, Ellen S.; Postlethwaite, Arnold; Schaeffer, Sandeford; Sawhney, Baljeet.

In: Cellular Immunology, Vol. 87, No. 2, 01.01.1984, p. 319-326.

Research output: Contribution to journalArticle

Kang, Ellen S. ; Postlethwaite, Arnold ; Schaeffer, Sandeford ; Sawhney, Baljeet. / Endogenous surface phosphorylation reactions and ectokinase activity in the guinea pig T lymphocyte. In: Cellular Immunology. 1984 ; Vol. 87, No. 2. pp. 319-326.
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