Endogenous type C viral gene expression in cultures of fetal diploid baboon cells treated with 5′-bromodeoxyuridine1,2

George Lavelle, Stephen Kennel, Linda J. Foote

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cultures of fetal diploid baboon fibroblasts treated with 5-bromodeoxyuridine synthesized protein antigenically related to baboon endogenous type C viral gag gene product, p28. Cellular immunofluorescence studies revealed induction of viral p28 in greater than 10% of treated cells in two baboon cell lines. Radioimmunoassays detected p28 antigenic specificities indistinguishable from those of purified virus [M7 strain of baboon endogenous virus (BEV)]. However, viral RNA-dependent DNA polymerase was not detected in culture fluids, and infectious virus was rarely recovered by cocultivation with susceptible heterologous cells. Extracellular particles containing p28 were not readily detected, further indicating that viral gag gene-coded proteins were synthesized independently of whole virus. Normal cultures of the same baboon cells exhibited endogenous expression of a glycoprotein antigenically related to BEV gp70, suggesting differential regulation of the endogenous gag and env gene-coded products. Baboon cell cultures exogenously infected with BEV produced extracellular particles having viral p28 and gp70 as measured by radioimmunoassays of culture fluids. Virus-infected cultures expressed about 0.01% of total lysate protein as p28, whereas bromodeoxyuridine-treated cultures expressed about 0.001% of their protein as p28. Since induced cultures have about 10% positive cells versus close to 100% for infected cultures, the amount of p28 per producing cell was about the same in both cell populations.

Original languageEnglish (US)
Pages (from-to)427-435
Number of pages9
JournalVirology
Volume110
Issue number2
DOIs
StatePublished - Apr 30 1981

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Viral Genes
Papio
Diploidy
Gene Expression
Viruses
gag Genes
Bromodeoxyuridine
Radioimmunoassay
Proteins
env Gene Products
gag Gene Products
RNA-Directed DNA Polymerase
Viral RNA
Viral Proteins
Coculture Techniques
Virion
Fluorescent Antibody Technique
Epitopes
Glycoproteins
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Endogenous type C viral gene expression in cultures of fetal diploid baboon cells treated with 5′-bromodeoxyuridine1,2 . / Lavelle, George; Kennel, Stephen; Foote, Linda J.

In: Virology, Vol. 110, No. 2, 30.04.1981, p. 427-435.

Research output: Contribution to journalArticle

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abstract = "Cultures of fetal diploid baboon fibroblasts treated with 5-bromodeoxyuridine synthesized protein antigenically related to baboon endogenous type C viral gag gene product, p28. Cellular immunofluorescence studies revealed induction of viral p28 in greater than 10{\%} of treated cells in two baboon cell lines. Radioimmunoassays detected p28 antigenic specificities indistinguishable from those of purified virus [M7 strain of baboon endogenous virus (BEV)]. However, viral RNA-dependent DNA polymerase was not detected in culture fluids, and infectious virus was rarely recovered by cocultivation with susceptible heterologous cells. Extracellular particles containing p28 were not readily detected, further indicating that viral gag gene-coded proteins were synthesized independently of whole virus. Normal cultures of the same baboon cells exhibited endogenous expression of a glycoprotein antigenically related to BEV gp70, suggesting differential regulation of the endogenous gag and env gene-coded products. Baboon cell cultures exogenously infected with BEV produced extracellular particles having viral p28 and gp70 as measured by radioimmunoassays of culture fluids. Virus-infected cultures expressed about 0.01{\%} of total lysate protein as p28, whereas bromodeoxyuridine-treated cultures expressed about 0.001{\%} of their protein as p28. Since induced cultures have about 10{\%} positive cells versus close to 100{\%} for infected cultures, the amount of p28 per producing cell was about the same in both cell populations.",
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