Endothelial barrier function

Regulation by shear stress and protein kinase C

C. M. Waters

Research output: Contribution to journalArticle

Abstract

Using endothelial cells (EC) cultured on porous microcarrier beads, we have measured the permeability of bovine pulmonary artery EC monolayers exposed to shear stress in a column chromatography system. We previously demonstrated that EC monolayer permeability was dependent upon the level of fluid shear stress to which the cells were exposed (J. Cell. Physiol., 1996). Since EC barrier function is in part regulated by bonds between junctional proteins and cytoskeletal components, we hypothesized that the maintenance of the EC barrier under shear stress may be a dynamic process which involves protein kinase C (PKC). We investigated the effect of PKC inhibition on endothelial permeability under static conditions and under 4.7 dynes/cm2 of shear stress using the PKC inhibitor calphostin C (calC). In the static (Transwell) system, the permeability to sodium fluorescein (NaFlsc, M.W. =342) was not affected by calC (control: 2.5 ± 0.3; calC: 23 ± 06; mean ± SEM × 10-5 cm/s, n=10). However, when EC's on microcarrier beads were exposed to shear stress, inhibition of PKC stimulated a steady increase in permeability which was significantly different (p<.05) from baseline approximately 1 hr after exposure to calC (14.0 ± 3.9 to 30.3 ± 8.4 × 10-5 cm/s; n=5). Inhibition of PKC may affect the ability of the cell cytoskeleton to remodel and adjust to shear stress.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997
Externally publishedYes

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Heat-Shock Proteins
Protein Kinase C
Endothelial cells
Shear stress
Permeability
Endothelial Cells
Monolayers
Cytoskeletal Proteins
Protein C Inhibitor
Protein Kinase Inhibitors
Column chromatography
Cytoskeleton
Fluorescein
Pulmonary Artery
Chromatography
Maintenance
calphostin C
Scanning electron microscopy
Fluids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Endothelial barrier function : Regulation by shear stress and protein kinase C. / Waters, C. M.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997.

Research output: Contribution to journalArticle

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