Endothelial cell induced modulation of cardiac fibroblast collagen metabolism

Eduardo Guarda, Paul R. Myers, Christian G. Brilla, Suresh C. Tyagi, Karl Weber

Research output: Contribution to journalArticle

Abstract

Objective: The aim was to examine the influence of vascular endothelial cells on rat cardiac fibroblast collagen synthesis and collagenase activity under co-culture conditions, and to determine whether angiotensin II or aldosterone influence endothelial cell induced modulation of fibroblast collagen metabolism. Methods: Bovine aortic endothelial cells were grown to confluence on microporous membrane inserts and then co-cultured with cardiac fibroblasts obtained from adult rats, previously grown to confluence in 35 mm dishes supplemented with 0.4% fetal bovine serum. Collagen synthesis was measured by 3H-proline incorporation. Experiments were also conducted under similar conditions to quantitate collagenase activity by zymography in conditioned medium from fibroblasts co-cultured with endothelial cells. Results: After 48 h co-culture, there was a 1.9-fold increase in fibroblast collagen synthesis when compared to fibroblasts alone (p<0.001). Aldosterone (10-8 M) or angiotensin II (10-7 M) added to endothelial cells did not increase fibroblast collagen synthesis over co-cultures alone. Neither the aldosterone receptor antagonist spironolactone (10-8 M) nor type I (DuP 753, 10-8 M) or type II (PD 123319, 10-8 M) angiotensin II receptor antagonists altered fibroblast collagen synthesis in co-cultures. A significant increment in collagenase activity was observed in co-cultured fibroblasts relative to collagenase activity of fibroblasts alone. Conclusions: Endothelial cells modulate both cardiac fibroblast collagen synthesis and degradation. The nature of the responsible signal(s) remains to be defined, but does not appear to be mediated or regulated by angiotensin II or aldosterone.Cardiovascular Research 1993;27:1004-1008.

Original languageEnglish (US)
Pages (from-to)1004-1008
Number of pages5
JournalCardiovascular research
Volume27
Issue number6
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Collagen
Endothelial Cells
Fibroblasts
Coculture Techniques
Collagenases
Aldosterone
Angiotensin II
Matrix Metalloproteinase 8
Mineralocorticoid Receptor Antagonists
Spironolactone
Losartan
Angiotensin Receptor Antagonists
Conditioned Culture Medium
Proline
Membranes
Serum
Research

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Endothelial cell induced modulation of cardiac fibroblast collagen metabolism. / Guarda, Eduardo; Myers, Paul R.; Brilla, Christian G.; Tyagi, Suresh C.; Weber, Karl.

In: Cardiovascular research, Vol. 27, No. 6, 01.01.1993, p. 1004-1008.

Research output: Contribution to journalArticle

Guarda, Eduardo ; Myers, Paul R. ; Brilla, Christian G. ; Tyagi, Suresh C. ; Weber, Karl. / Endothelial cell induced modulation of cardiac fibroblast collagen metabolism. In: Cardiovascular research. 1993 ; Vol. 27, No. 6. pp. 1004-1008.
@article{d6d742aae85544df9c3d1f7b5fe1e41e,
title = "Endothelial cell induced modulation of cardiac fibroblast collagen metabolism",
abstract = "Objective: The aim was to examine the influence of vascular endothelial cells on rat cardiac fibroblast collagen synthesis and collagenase activity under co-culture conditions, and to determine whether angiotensin II or aldosterone influence endothelial cell induced modulation of fibroblast collagen metabolism. Methods: Bovine aortic endothelial cells were grown to confluence on microporous membrane inserts and then co-cultured with cardiac fibroblasts obtained from adult rats, previously grown to confluence in 35 mm dishes supplemented with 0.4{\%} fetal bovine serum. Collagen synthesis was measured by 3H-proline incorporation. Experiments were also conducted under similar conditions to quantitate collagenase activity by zymography in conditioned medium from fibroblasts co-cultured with endothelial cells. Results: After 48 h co-culture, there was a 1.9-fold increase in fibroblast collagen synthesis when compared to fibroblasts alone (p<0.001). Aldosterone (10-8 M) or angiotensin II (10-7 M) added to endothelial cells did not increase fibroblast collagen synthesis over co-cultures alone. Neither the aldosterone receptor antagonist spironolactone (10-8 M) nor type I (DuP 753, 10-8 M) or type II (PD 123319, 10-8 M) angiotensin II receptor antagonists altered fibroblast collagen synthesis in co-cultures. A significant increment in collagenase activity was observed in co-cultured fibroblasts relative to collagenase activity of fibroblasts alone. Conclusions: Endothelial cells modulate both cardiac fibroblast collagen synthesis and degradation. The nature of the responsible signal(s) remains to be defined, but does not appear to be mediated or regulated by angiotensin II or aldosterone.Cardiovascular Research 1993;27:1004-1008.",
author = "Eduardo Guarda and Myers, {Paul R.} and Brilla, {Christian G.} and Tyagi, {Suresh C.} and Karl Weber",
year = "1993",
month = "1",
day = "1",
doi = "10.1093/cvr/27.6.1004",
language = "English (US)",
volume = "27",
pages = "1004--1008",
journal = "Cardiovascular Research",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Endothelial cell induced modulation of cardiac fibroblast collagen metabolism

AU - Guarda, Eduardo

AU - Myers, Paul R.

AU - Brilla, Christian G.

AU - Tyagi, Suresh C.

AU - Weber, Karl

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Objective: The aim was to examine the influence of vascular endothelial cells on rat cardiac fibroblast collagen synthesis and collagenase activity under co-culture conditions, and to determine whether angiotensin II or aldosterone influence endothelial cell induced modulation of fibroblast collagen metabolism. Methods: Bovine aortic endothelial cells were grown to confluence on microporous membrane inserts and then co-cultured with cardiac fibroblasts obtained from adult rats, previously grown to confluence in 35 mm dishes supplemented with 0.4% fetal bovine serum. Collagen synthesis was measured by 3H-proline incorporation. Experiments were also conducted under similar conditions to quantitate collagenase activity by zymography in conditioned medium from fibroblasts co-cultured with endothelial cells. Results: After 48 h co-culture, there was a 1.9-fold increase in fibroblast collagen synthesis when compared to fibroblasts alone (p<0.001). Aldosterone (10-8 M) or angiotensin II (10-7 M) added to endothelial cells did not increase fibroblast collagen synthesis over co-cultures alone. Neither the aldosterone receptor antagonist spironolactone (10-8 M) nor type I (DuP 753, 10-8 M) or type II (PD 123319, 10-8 M) angiotensin II receptor antagonists altered fibroblast collagen synthesis in co-cultures. A significant increment in collagenase activity was observed in co-cultured fibroblasts relative to collagenase activity of fibroblasts alone. Conclusions: Endothelial cells modulate both cardiac fibroblast collagen synthesis and degradation. The nature of the responsible signal(s) remains to be defined, but does not appear to be mediated or regulated by angiotensin II or aldosterone.Cardiovascular Research 1993;27:1004-1008.

AB - Objective: The aim was to examine the influence of vascular endothelial cells on rat cardiac fibroblast collagen synthesis and collagenase activity under co-culture conditions, and to determine whether angiotensin II or aldosterone influence endothelial cell induced modulation of fibroblast collagen metabolism. Methods: Bovine aortic endothelial cells were grown to confluence on microporous membrane inserts and then co-cultured with cardiac fibroblasts obtained from adult rats, previously grown to confluence in 35 mm dishes supplemented with 0.4% fetal bovine serum. Collagen synthesis was measured by 3H-proline incorporation. Experiments were also conducted under similar conditions to quantitate collagenase activity by zymography in conditioned medium from fibroblasts co-cultured with endothelial cells. Results: After 48 h co-culture, there was a 1.9-fold increase in fibroblast collagen synthesis when compared to fibroblasts alone (p<0.001). Aldosterone (10-8 M) or angiotensin II (10-7 M) added to endothelial cells did not increase fibroblast collagen synthesis over co-cultures alone. Neither the aldosterone receptor antagonist spironolactone (10-8 M) nor type I (DuP 753, 10-8 M) or type II (PD 123319, 10-8 M) angiotensin II receptor antagonists altered fibroblast collagen synthesis in co-cultures. A significant increment in collagenase activity was observed in co-cultured fibroblasts relative to collagenase activity of fibroblasts alone. Conclusions: Endothelial cells modulate both cardiac fibroblast collagen synthesis and degradation. The nature of the responsible signal(s) remains to be defined, but does not appear to be mediated or regulated by angiotensin II or aldosterone.Cardiovascular Research 1993;27:1004-1008.

UR - http://www.scopus.com/inward/record.url?scp=85047677462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047677462&partnerID=8YFLogxK

U2 - 10.1093/cvr/27.6.1004

DO - 10.1093/cvr/27.6.1004

M3 - Article

VL - 27

SP - 1004

EP - 1008

JO - Cardiovascular Research

JF - Cardiovascular Research

SN - 0008-6363

IS - 6

ER -