Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction

Nami Nakagomi, Takayuki Nakagomi, Shuji Kubo, Akiko Nakano-Doi, Orie Saino, Masashi Takata, Hiroo Yoshikawa, David Stern, Tomohiro Matsuyama, Akihiko Taguchi

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Transplantation of neural stem cells (NSCs) has been proposed as a therapy for a range of neurological disorders. To realize the potential of this approach, it is essential to control survival, proliferation, migration, and differentiation of NSCs after transplantation. NSCs are regulated in vivo, at least in part, by their specialized microenvironment or "niche." In the adult central nervous system, neurogenic regions, such as the subventricular and subgranular zones, include NSCs residing in a vascular niche with endothelial cells. Although there is accumulating evidence that endothelial cells promote proliferation of NSCs in vitro, there is no description of their impact on transplanted NSCs. In this study, we grafted cortex-derived stroke-induced neural stem/progenitor cells, obtained from adult mice, onto poststroke cortex in the presence or absence of endothelial cells, and compared survival, proliferation, and neuronal differentiation of the neural precursors in vivo. Cotransplantation of endothelial cells and neural stem/progenitor cells increased survival and proliferation of ischemia-induced neural stem/progenitor cells and also accelerated neuronal differentiation compared with transplantation of neural precursors alone. These data indicate that reconstitution of elements in the vascular niche enhances transplantation of adult neural progenitor cells.

Original languageEnglish (US)
Pages (from-to)2185-2195
Number of pages11
JournalStem Cells
Volume27
Issue number9
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

Fingerprint

Neural Stem Cells
Cerebral Infarction
Cell Survival
Stem Cells
Ischemia
Endothelial Cells
Transplantation
Blood Vessels
Cell Proliferation
Lateral Ventricles
Stem Cell Transplantation
Nervous System Diseases
Central Nervous System
Stroke

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction. / Nakagomi, Nami; Nakagomi, Takayuki; Kubo, Shuji; Nakano-Doi, Akiko; Saino, Orie; Takata, Masashi; Yoshikawa, Hiroo; Stern, David; Matsuyama, Tomohiro; Taguchi, Akihiko.

In: Stem Cells, Vol. 27, No. 9, 01.09.2009, p. 2185-2195.

Research output: Contribution to journalArticle

Nakagomi, N, Nakagomi, T, Kubo, S, Nakano-Doi, A, Saino, O, Takata, M, Yoshikawa, H, Stern, D, Matsuyama, T & Taguchi, A 2009, 'Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction', Stem Cells, vol. 27, no. 9, pp. 2185-2195. https://doi.org/10.1002/stem.161
Nakagomi, Nami ; Nakagomi, Takayuki ; Kubo, Shuji ; Nakano-Doi, Akiko ; Saino, Orie ; Takata, Masashi ; Yoshikawa, Hiroo ; Stern, David ; Matsuyama, Tomohiro ; Taguchi, Akihiko. / Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction. In: Stem Cells. 2009 ; Vol. 27, No. 9. pp. 2185-2195.
@article{c9f0e665f98e4c1595551f3ada542bfe,
title = "Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction",
abstract = "Transplantation of neural stem cells (NSCs) has been proposed as a therapy for a range of neurological disorders. To realize the potential of this approach, it is essential to control survival, proliferation, migration, and differentiation of NSCs after transplantation. NSCs are regulated in vivo, at least in part, by their specialized microenvironment or {"}niche.{"} In the adult central nervous system, neurogenic regions, such as the subventricular and subgranular zones, include NSCs residing in a vascular niche with endothelial cells. Although there is accumulating evidence that endothelial cells promote proliferation of NSCs in vitro, there is no description of their impact on transplanted NSCs. In this study, we grafted cortex-derived stroke-induced neural stem/progenitor cells, obtained from adult mice, onto poststroke cortex in the presence or absence of endothelial cells, and compared survival, proliferation, and neuronal differentiation of the neural precursors in vivo. Cotransplantation of endothelial cells and neural stem/progenitor cells increased survival and proliferation of ischemia-induced neural stem/progenitor cells and also accelerated neuronal differentiation compared with transplantation of neural precursors alone. These data indicate that reconstitution of elements in the vascular niche enhances transplantation of adult neural progenitor cells.",
author = "Nami Nakagomi and Takayuki Nakagomi and Shuji Kubo and Akiko Nakano-Doi and Orie Saino and Masashi Takata and Hiroo Yoshikawa and David Stern and Tomohiro Matsuyama and Akihiko Taguchi",
year = "2009",
month = "9",
day = "1",
doi = "10.1002/stem.161",
language = "English (US)",
volume = "27",
pages = "2185--2195",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "9",

}

TY - JOUR

T1 - Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction

AU - Nakagomi, Nami

AU - Nakagomi, Takayuki

AU - Kubo, Shuji

AU - Nakano-Doi, Akiko

AU - Saino, Orie

AU - Takata, Masashi

AU - Yoshikawa, Hiroo

AU - Stern, David

AU - Matsuyama, Tomohiro

AU - Taguchi, Akihiko

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Transplantation of neural stem cells (NSCs) has been proposed as a therapy for a range of neurological disorders. To realize the potential of this approach, it is essential to control survival, proliferation, migration, and differentiation of NSCs after transplantation. NSCs are regulated in vivo, at least in part, by their specialized microenvironment or "niche." In the adult central nervous system, neurogenic regions, such as the subventricular and subgranular zones, include NSCs residing in a vascular niche with endothelial cells. Although there is accumulating evidence that endothelial cells promote proliferation of NSCs in vitro, there is no description of their impact on transplanted NSCs. In this study, we grafted cortex-derived stroke-induced neural stem/progenitor cells, obtained from adult mice, onto poststroke cortex in the presence or absence of endothelial cells, and compared survival, proliferation, and neuronal differentiation of the neural precursors in vivo. Cotransplantation of endothelial cells and neural stem/progenitor cells increased survival and proliferation of ischemia-induced neural stem/progenitor cells and also accelerated neuronal differentiation compared with transplantation of neural precursors alone. These data indicate that reconstitution of elements in the vascular niche enhances transplantation of adult neural progenitor cells.

AB - Transplantation of neural stem cells (NSCs) has been proposed as a therapy for a range of neurological disorders. To realize the potential of this approach, it is essential to control survival, proliferation, migration, and differentiation of NSCs after transplantation. NSCs are regulated in vivo, at least in part, by their specialized microenvironment or "niche." In the adult central nervous system, neurogenic regions, such as the subventricular and subgranular zones, include NSCs residing in a vascular niche with endothelial cells. Although there is accumulating evidence that endothelial cells promote proliferation of NSCs in vitro, there is no description of their impact on transplanted NSCs. In this study, we grafted cortex-derived stroke-induced neural stem/progenitor cells, obtained from adult mice, onto poststroke cortex in the presence or absence of endothelial cells, and compared survival, proliferation, and neuronal differentiation of the neural precursors in vivo. Cotransplantation of endothelial cells and neural stem/progenitor cells increased survival and proliferation of ischemia-induced neural stem/progenitor cells and also accelerated neuronal differentiation compared with transplantation of neural precursors alone. These data indicate that reconstitution of elements in the vascular niche enhances transplantation of adult neural progenitor cells.

UR - http://www.scopus.com/inward/record.url?scp=70349847390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349847390&partnerID=8YFLogxK

U2 - 10.1002/stem.161

DO - 10.1002/stem.161

M3 - Article

VL - 27

SP - 2185

EP - 2195

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 9

ER -