Endothelial nitric oxide synthase genotypes in the etiology of retinopathy of prematurity in premature infants

Krishna Yanamandra, Dawn Napper, Arun Pramanik, Joseph A. Bocchini, Ramasubbareddy Dhanireddy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Retinopathy of Prematurity (ROP) is a vasoproliferative disorder affecting preterm infants leading to visual impairment. ROP is more common in Caucasians than African Americans. Very low birth weight infants have immature retinas and are susceptible to ROP. Because of differences in individual responses to the treatment, various genetic factors have been looked into to understand the etiology of ROP. Endothelial nitric oxide (eNO) serves as a vasodilator, relaxes smooth muscle, prevents platelet aggregation, and facilitates improved blood flow and vascular tonicity. Mutant eNO synthase (eNOS) genotypes result in reduced nitric oxide levels by decreasing enzyme activity. Since eNO affects vasculature and ROP is a vascular disease, the present investigation was aimed at studying the association of genotypes with ROP. Methods: Two eNOS gene single nucleotide polymorphisms (SNPs) (T-786C, and G894T) were studied by microplate-Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP PCR) method. Genotypes were studied in 146 premature infants. Results: The present data showed significant differences in the baseline gene frequencies between Caucasians and African Americans. ROP patients displayed 3-fold higher frequencies of mutant -786C and 894T alleles in both ethnicities compared to respective controls. Conclusions: The present data suggest ethnic stratification of genotypes. Mutant -786C and 894T alleles are significant risk factors in the development of ROP, and suggest a strong association between eNOS polymorphisms and the disease. It is interesting to know if a larger dataset of ROP patients can confirm our initial findings.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalOphthalmic Genetics
Volume31
Issue number4
DOIs
StatePublished - Dec 1 2010

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Retinopathy of Prematurity
Nitric Oxide Synthase Type III
Premature Infants
Genotype
Nitric Oxide
African Americans
Alleles
Very Low Birth Weight Infant
Vision Disorders
Vasodilator Agents
Vascular Diseases
Platelet Aggregation
Gene Frequency
Individuality
Restriction Fragment Length Polymorphisms
Single Nucleotide Polymorphism
Smooth Muscle
Blood Vessels
Retina
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Pediatrics, Perinatology, and Child Health
  • Genetics(clinical)

Cite this

Endothelial nitric oxide synthase genotypes in the etiology of retinopathy of prematurity in premature infants. / Yanamandra, Krishna; Napper, Dawn; Pramanik, Arun; Bocchini, Joseph A.; Dhanireddy, Ramasubbareddy.

In: Ophthalmic Genetics, Vol. 31, No. 4, 01.12.2010, p. 173-177.

Research output: Contribution to journalArticle

Yanamandra, Krishna ; Napper, Dawn ; Pramanik, Arun ; Bocchini, Joseph A. ; Dhanireddy, Ramasubbareddy. / Endothelial nitric oxide synthase genotypes in the etiology of retinopathy of prematurity in premature infants. In: Ophthalmic Genetics. 2010 ; Vol. 31, No. 4. pp. 173-177.
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