Endothelin-1 production from endothelial cells

Role of protein kinase C

M. A. Yakubu, Charles Leffler

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Elevated ET-1 can be produced following subarachnoid hematoma and ET-1 may be involved in hematoma potentiated cerebral vasoconstriction. This study was designed to investigate the potential contributiuons of serotonin (5-HT), lysophosphatidic acid (LPA) and thromboxane receptor agonist (U-46619) in ET-1 production using piglet cerebral microvascularendothelial cells in culture. Confluent cells were treated with 5-HT (10-4 M), LPA (10-4 M), and U-46619 (10-5 M) for 4 hours at 37°C. ET-1 in the sample was determined using an ET-1 sensitive radioimmunoassay. ET-1 was significantly elevated from 175±34 fmol/mL in control to 356±63 (5-HT), 430±59 (LPA), and 451±57 (U-46619) fmol/mL. Staurosporine (10-6 M), a PKC inhibitor blocked stimulation in response to 5-HT (140±21), LPA (84±7), and U-16619 (125±20) fmol/mL. In conclusion, several factors that are produced by hemorrhaged blood can stimulate ET-1 production by cerebral microvascular endothelial cells via mechanisms involving protein kinase C. Elevated ET-1 in conjunction with cerebral hematoma could contribute to enhanced cerebral vasoconstriction.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

endothelins
Endothelial cells
protein kinase C
Endothelin-1
serotonin
Protein Kinase C
endothelial cells
Serotonin
Endothelial Cells
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
hematoma
Hematoma
vasoconstriction
acids
Vasoconstriction
Lysophosphatidic Acid Receptors
Thromboxane Receptors
thromboxanes
Staurosporine
radioimmunoassays

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Endothelin-1 production from endothelial cells : Role of protein kinase C. / Yakubu, M. A.; Leffler, Charles.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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