Endotoxin potentiates lung injury in cerulein-induced pancreatitis

Keith Gray, Misho O. Simovic, William C. Chapman, Timothy S. Blackwell, John W. Christman, Addison K. May, Kelly S. Parman, Steven C. Stain

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Background: In this study we examine the effect of endotoxin (lipopolysaccharide) on lung injury in the setting of acute pancreatitis (AP). Methods: Twelve hourly injections of cerulein (50 μg/kg/h) were used to induce pancreatitis in mice. Intraperitoneal lipopolysaccharide (LPS [6 mg/kg]) was administered 24 hours after the initial cerulein injection. Twenty-four hours after LPS injection, myeloperoxidase (MPO) activity, nuclear factor (NF)-κB activation, and tumor necrosis factor (TNF)-α, interleukin (IL)-6, and chemokines MIP-2 and KC levels were measured in pancreas, liver, and lung tissues. Four groups of mice were studied: cerulein-LPS, cerulein-saline, saline-LPS, and saline-saline treated mice. Results: Elevated serum lipase confirmed pancreatitis in cerulein treated mice. Lung MPO activity was significantly increased in the cerulein-LPS group. NF-κB was activated in the liver but not in pancreas and lung tissue. Chemokines MIP-2 and KC were elevated in pancreatic tissue only. Conclusions: These findings suggest that gram-negative infections may be an important predisposition for the development of adult respiratory distress syndrome in the setting of AP and that hepatic NF-κB may mediate multisystem injury.

Original languageEnglish (US)
Pages (from-to)526-530
Number of pages5
JournalAmerican Journal of Surgery
Volume186
Issue number5
DOIs
StatePublished - Jan 1 2003

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Ceruletide
Lung Injury
Endotoxins
Pancreatitis
Chemokine CXCL2
Lung
Peroxidase
Injections
Lipopolysaccharides
Pancreas
Liver
Adult Respiratory Distress Syndrome
Lipase
Interleukin-6
Tumor Necrosis Factor-alpha
Wounds and Injuries
Infection
Serum

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Gray, K., Simovic, M. O., Chapman, W. C., Blackwell, T. S., Christman, J. W., May, A. K., ... Stain, S. C. (2003). Endotoxin potentiates lung injury in cerulein-induced pancreatitis. American Journal of Surgery, 186(5), 526-530. https://doi.org/10.1016/j.amjsurg.2003.07.010

Endotoxin potentiates lung injury in cerulein-induced pancreatitis. / Gray, Keith; Simovic, Misho O.; Chapman, William C.; Blackwell, Timothy S.; Christman, John W.; May, Addison K.; Parman, Kelly S.; Stain, Steven C.

In: American Journal of Surgery, Vol. 186, No. 5, 01.01.2003, p. 526-530.

Research output: Contribution to journalArticle

Gray, K, Simovic, MO, Chapman, WC, Blackwell, TS, Christman, JW, May, AK, Parman, KS & Stain, SC 2003, 'Endotoxin potentiates lung injury in cerulein-induced pancreatitis', American Journal of Surgery, vol. 186, no. 5, pp. 526-530. https://doi.org/10.1016/j.amjsurg.2003.07.010
Gray K, Simovic MO, Chapman WC, Blackwell TS, Christman JW, May AK et al. Endotoxin potentiates lung injury in cerulein-induced pancreatitis. American Journal of Surgery. 2003 Jan 1;186(5):526-530. https://doi.org/10.1016/j.amjsurg.2003.07.010
Gray, Keith ; Simovic, Misho O. ; Chapman, William C. ; Blackwell, Timothy S. ; Christman, John W. ; May, Addison K. ; Parman, Kelly S. ; Stain, Steven C. / Endotoxin potentiates lung injury in cerulein-induced pancreatitis. In: American Journal of Surgery. 2003 ; Vol. 186, No. 5. pp. 526-530.
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