Enhanced extracellular growth of Staphylococcus aureus in the presence of selected linear peptide fragments of human interleukin (IL)-1β and IL-1 receptor antagonist

Siva Kanangat, Michael S. Bronze, Gianfranco Meduri, Arnold Postlethwaite, Frankie Stentz, Elizabeth Tolley, Dennis Schaberg

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Replication of Staphylococcus aureus is significantly enhanced in the presence of recombinant interleukin (IL)-1β. In this study, specific binding of IL-1β to the surface of S. aureus significantly increased growth of S. aureus in the presence of IL-1β and IL-1ra in a concentration-dependent manner. Although IL-1ra enhanced the growth of S. aureus, there was a significant reduction in IL-1β-mediated growth enhancement of S. aureus when 25-fold excess amounts of IL-1ra (in comparison with the IL-1β concentration) were present in the culture medium. Thus, IL-1β may influence the growth of S. aureus through a receptor-mediated event. By using 5 linear peptides spanning limited regions of IL-1β, the growth-promoting regions were localized to amino acid residues 118-147 and 208-240. These results build on the newly evolved concept of direct interactions between the soluble mediators of inflammation and infectious agents.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalJournal of Infectious Diseases
Volume183
Issue number1
DOIs
StatePublished - Jan 1 2001

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Peptide Fragments
Interleukin-1 Receptors
Interleukin-1
Staphylococcus aureus
Growth
Interleukins
Inflammation Mediators
Culture Media
Amino Acids
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

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title = "Enhanced extracellular growth of Staphylococcus aureus in the presence of selected linear peptide fragments of human interleukin (IL)-1β and IL-1 receptor antagonist",
abstract = "Replication of Staphylococcus aureus is significantly enhanced in the presence of recombinant interleukin (IL)-1β. In this study, specific binding of IL-1β to the surface of S. aureus significantly increased growth of S. aureus in the presence of IL-1β and IL-1ra in a concentration-dependent manner. Although IL-1ra enhanced the growth of S. aureus, there was a significant reduction in IL-1β-mediated growth enhancement of S. aureus when 25-fold excess amounts of IL-1ra (in comparison with the IL-1β concentration) were present in the culture medium. Thus, IL-1β may influence the growth of S. aureus through a receptor-mediated event. By using 5 linear peptides spanning limited regions of IL-1β, the growth-promoting regions were localized to amino acid residues 118-147 and 208-240. These results build on the newly evolved concept of direct interactions between the soluble mediators of inflammation and infectious agents.",
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T1 - Enhanced extracellular growth of Staphylococcus aureus in the presence of selected linear peptide fragments of human interleukin (IL)-1β and IL-1 receptor antagonist

AU - Kanangat, Siva

AU - Bronze, Michael S.

AU - Meduri, Gianfranco

AU - Postlethwaite, Arnold

AU - Stentz, Frankie

AU - Tolley, Elizabeth

AU - Schaberg, Dennis

PY - 2001/1/1

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N2 - Replication of Staphylococcus aureus is significantly enhanced in the presence of recombinant interleukin (IL)-1β. In this study, specific binding of IL-1β to the surface of S. aureus significantly increased growth of S. aureus in the presence of IL-1β and IL-1ra in a concentration-dependent manner. Although IL-1ra enhanced the growth of S. aureus, there was a significant reduction in IL-1β-mediated growth enhancement of S. aureus when 25-fold excess amounts of IL-1ra (in comparison with the IL-1β concentration) were present in the culture medium. Thus, IL-1β may influence the growth of S. aureus through a receptor-mediated event. By using 5 linear peptides spanning limited regions of IL-1β, the growth-promoting regions were localized to amino acid residues 118-147 and 208-240. These results build on the newly evolved concept of direct interactions between the soluble mediators of inflammation and infectious agents.

AB - Replication of Staphylococcus aureus is significantly enhanced in the presence of recombinant interleukin (IL)-1β. In this study, specific binding of IL-1β to the surface of S. aureus significantly increased growth of S. aureus in the presence of IL-1β and IL-1ra in a concentration-dependent manner. Although IL-1ra enhanced the growth of S. aureus, there was a significant reduction in IL-1β-mediated growth enhancement of S. aureus when 25-fold excess amounts of IL-1ra (in comparison with the IL-1β concentration) were present in the culture medium. Thus, IL-1β may influence the growth of S. aureus through a receptor-mediated event. By using 5 linear peptides spanning limited regions of IL-1β, the growth-promoting regions were localized to amino acid residues 118-147 and 208-240. These results build on the newly evolved concept of direct interactions between the soluble mediators of inflammation and infectious agents.

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