Enhanced pial arteriolar sensitivity to bioactive agents following exposure to endothelin-1

Momoh A. Yakubu, Charles Leffler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Effects of prior exposure of pial arterioles to endothelin-1 (ET-1) (10-9 M) on the constriction induced by the by-products of hemolyzed blood (5-HT, LTC4, LPA, and thromboxane analog U-46619) were examined. Piglets (age: 1-3 d) anesthetized with a mixture of ketamine hydrochloride and acepromazine were implanted with cranial windows, and anesthesia was maintained with α-chloralose. Topical applications of the by-products of hemolyzed blood mildly constricted pial arterioles. Following prior exposure of the microvessels to ET-1, application of the by-products of hemolyzed blood produced significantly potentiated and long-lasting constrictions compared to the controls. In another experiment, pretreatment of pial arterioles with U-46619 (10-8 M) also potentiated the constriction induced by ET-1. The constriction produced was fast and longer-lasting. Thus, these data show that by-products of hemolyzed blood, though not potent vasoconstrictors per se, potently constricted pial arterioles in the presence of ET-1. The same agents in the CSF can also potentiate constriction induced by ET-1. Hence, by-products of hemolyzed blood may play a significant role in the initiation and maintenance of cerebral arterial narrowing observed following intracranial bleeding.

Original languageEnglish (US)
Pages (from-to)307-316
Number of pages10
JournalLife Sciences
Volume66
Issue number4
DOIs
StatePublished - Dec 14 1999

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Endothelin-1
Constriction
Byproducts
Arterioles
Blood
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Acepromazine
Leukotriene C4
Chloralose
Thromboxanes
Ketamine
Vasoconstrictor Agents
Microvessels
Serotonin
Anesthesia
Maintenance
Hemorrhage
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Enhanced pial arteriolar sensitivity to bioactive agents following exposure to endothelin-1. / Yakubu, Momoh A.; Leffler, Charles.

In: Life Sciences, Vol. 66, No. 4, 14.12.1999, p. 307-316.

Research output: Contribution to journalArticle

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