Enhanced proinflammatory cytokine activity during experimental bluetongue virus-1 infection in Indian native sheep

Rudragouda Channappanavar, K. P. Singh, R. Singh, C. S. Umeshappa, S. L. Ingale, A. B. Pandey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bluetongue disease has been causing variable morbidity and mortality in sheep in India and other countries of the world. The experimental infection with Indian BTV-1 strain induced mild to sub-acute infection in native sheep. There were low induction of IL-12, IFN-γ and TNF-α cytokine mRNA expressions determined by real time RT-PCR in draining lymph nodes (DLN), spleen, and PBMCs during the initial stages of infection (8 days post inoculation, DPI) and higher around 15 DPI. The reduced pro-inflammatory cytokine (IFN-γ and TNF-α) responses during the initial stage of infection (8 DPI) was also accompanied by similarly decreased T-cell populations and overt clinical symptoms. Later up regulation of these cytokines and substantial increase in the proportion of CD8 + T-cells occurred with reduction of clinical signs and disappearance of BTV-1 antigen from tissues as determined by immunohistochemistry and RT-PCR. Thus there is definite involvement of pro-inflammatory cytokines and CD8 + T cell activity in disease induced by BTV-1 strain.

Original languageEnglish (US)
Pages (from-to)485-492
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume145
Issue number1-2
DOIs
StatePublished - Jan 15 2012
Externally publishedYes

Fingerprint

Bluetongue virus
Virus Diseases
Sheep
cytokines
Cytokines
sheep
T-lymphocytes
Infection
T-Lymphocytes
infection
reverse transcriptase polymerase chain reaction
Bluetongue
bluetongue
interleukin-12
Interleukin-12
mononuclear leukocytes
signs and symptoms (animals and humans)
immunohistochemistry
lymph nodes
morbidity

All Science Journal Classification (ASJC) codes

  • Immunology
  • veterinary(all)

Cite this

Enhanced proinflammatory cytokine activity during experimental bluetongue virus-1 infection in Indian native sheep. / Channappanavar, Rudragouda; Singh, K. P.; Singh, R.; Umeshappa, C. S.; Ingale, S. L.; Pandey, A. B.

In: Veterinary Immunology and Immunopathology, Vol. 145, No. 1-2, 15.01.2012, p. 485-492.

Research output: Contribution to journalArticle

Channappanavar, Rudragouda ; Singh, K. P. ; Singh, R. ; Umeshappa, C. S. ; Ingale, S. L. ; Pandey, A. B. / Enhanced proinflammatory cytokine activity during experimental bluetongue virus-1 infection in Indian native sheep. In: Veterinary Immunology and Immunopathology. 2012 ; Vol. 145, No. 1-2. pp. 485-492.
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