Enzyme-linked immunosorbent assay for variable region λ VI subgroup of light chain in serum

Method and results in normal subjects and patients with hyper-and hypogammaglobulinemia

Masahiro Abe, Tetsuya Goto, Shuji Ozaki, Masaaki Kosaka, Alan Solomon

Research output: Contribution to journalArticle

Abstract

We have established a simple, sensitive and reproducible sandwich enzyme-linked immunosorbent assay (ELISA) for quantitation of immunoglobulins of A VI subgroup (Ig λ VI) which has been of special interest by its amyloidogenicity. It used murine monoclonal antibodies specific for λ VI subgroup of variable region (VL) and for λ type of constant region (CL) of human immunoglobulin light chains. The A VI protein level and the percent distribution of A VI subgroup among total IgA proteins (λ VI/λ ratio) in serum were determined. Igλ VI proteins were contained in each of serum specimens tested. In serum specimens obtained from 44 normal subjects and 14 patients with polyclonal hypergammaglobulinemia and six patients with hypogammaglobulinemia, the concentration (mean±SD) of Igλ VI proteins was 78 to 432 (197±81), 218 to 1875 (648 ± 474) and 49 to 115 (85±25) μg/ ml and that of total IgA proteins was 2, 640 to 8, 300 (4, 712±1, 454), 8, 197 to 30, 000 (14, 837±7, 029) and 890 to 1, 800 (1312±340) μg/ml, and the A VI/λ ratio (mean±SD) was 1.2 to 9.4 (4.3±1.7), 2.1 to 6.4 (4.2±1.7) and 4.6 to 9.2 (6.5±1.6)%, respectively. The A VI/A ratio was strikingly higher than 10% in serum specimens from three patients with A Vl-related amyloidosis, which implied clinical and diagnostic availability of this ratio. In patients with rheumatoid arthritis, systemic lupus erythematosus and liver cirrhosis present- ing polyclonal hypergammaglobulinemia, λ VI proteins were contained at high concentration in serum, but no amyloid deposition was found in biopsied specimens. However, further study on more cases with polyclonal Igλ VI protein overproduction is required to elucidate amyloidogenicity of non-malignant Igλ VI proteins. The assays described here will facilitate the study on monoclonal and polyclonal nature of this unique λ VI subgroup in various disease conditions.

Original languageEnglish (US)
Pages (from-to)172-181
Number of pages10
JournalJapanese Journal of Clinical Immunology
Volume17
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Agammaglobulinemia
Enzyme-Linked Immunosorbent Assay
Light
Serum
Immunoglobulin A
Proteins
Hypergammaglobulinemia
Immunoglobulin Light Chains
Amyloidosis
Amyloid
Liver Cirrhosis
Systemic Lupus Erythematosus
Blood Proteins
Rheumatoid Arthritis
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Enzyme-linked immunosorbent assay for variable region λ VI subgroup of light chain in serum : Method and results in normal subjects and patients with hyper-and hypogammaglobulinemia. / Abe, Masahiro; Goto, Tetsuya; Ozaki, Shuji; Kosaka, Masaaki; Solomon, Alan.

In: Japanese Journal of Clinical Immunology, Vol. 17, No. 3, 01.01.1994, p. 172-181.

Research output: Contribution to journalArticle

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abstract = "We have established a simple, sensitive and reproducible sandwich enzyme-linked immunosorbent assay (ELISA) for quantitation of immunoglobulins of A VI subgroup (Ig λ VI) which has been of special interest by its amyloidogenicity. It used murine monoclonal antibodies specific for λ VI subgroup of variable region (VL) and for λ type of constant region (CL) of human immunoglobulin light chains. The A VI protein level and the percent distribution of A VI subgroup among total IgA proteins (λ VI/λ ratio) in serum were determined. Igλ VI proteins were contained in each of serum specimens tested. In serum specimens obtained from 44 normal subjects and 14 patients with polyclonal hypergammaglobulinemia and six patients with hypogammaglobulinemia, the concentration (mean±SD) of Igλ VI proteins was 78 to 432 (197±81), 218 to 1875 (648 ± 474) and 49 to 115 (85±25) μg/ ml and that of total IgA proteins was 2, 640 to 8, 300 (4, 712±1, 454), 8, 197 to 30, 000 (14, 837±7, 029) and 890 to 1, 800 (1312±340) μg/ml, and the A VI/λ ratio (mean±SD) was 1.2 to 9.4 (4.3±1.7), 2.1 to 6.4 (4.2±1.7) and 4.6 to 9.2 (6.5±1.6){\%}, respectively. The A VI/A ratio was strikingly higher than 10{\%} in serum specimens from three patients with A Vl-related amyloidosis, which implied clinical and diagnostic availability of this ratio. In patients with rheumatoid arthritis, systemic lupus erythematosus and liver cirrhosis present- ing polyclonal hypergammaglobulinemia, λ VI proteins were contained at high concentration in serum, but no amyloid deposition was found in biopsied specimens. However, further study on more cases with polyclonal Igλ VI protein overproduction is required to elucidate amyloidogenicity of non-malignant Igλ VI proteins. The assays described here will facilitate the study on monoclonal and polyclonal nature of this unique λ VI subgroup in various disease conditions.",
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