Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma

K. L. Denzler, S. C. Farmer, J. R. Crosby, M. Borchers, G. Cieslewicz, K. A. Larson, Stephania Cormier, N. A. Lee, J. J. Lee

Research output: Contribution to journalArticle

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Abstract

The relationship between eosinophils and the development of Ag-induced pulmonary pathologies, including airway hyper-responsiveness, was investigated using mice deficient for the secondary granule component, major basic protein-1 (mMBP-1). The loss of mMBP-1 had no effect on OVA-induced airway histopathologies or inflammatory cell recruitment. Lung function measurements of knockout mice demonstrated a generalized hyporeactivity to methacholine-induced airflow changes (relative to wild type); however, this baseline phenotype was observable only with methacholine; no relative airflow changes were observed in response to another nonspecific stimulus (serotonin). Moreover, OVA sensitization/aerosol challenge of wild-type and mMBP-1(-/-) mice resulted in identical dose-response changes to either methacholine or serotonin. Thus, the airway hyper-responsiveness in murine models of asthma occurs in the absence of mMBP-1.

Original languageEnglish (US)
Pages (from-to)5509-5517
Number of pages9
JournalJournal of Immunology
Volume165
Issue number10
DOIs
StatePublished - Nov 15 2000

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Eosinophil Major Basic Protein
Methacholine Chloride
Respiratory Hypersensitivity
Allergens
Asthma
Pathology
Serotonin
Lung
Aerosols
Eosinophils
Knockout Mice
Phenotype
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Denzler, K. L., Farmer, S. C., Crosby, J. R., Borchers, M., Cieslewicz, G., Larson, K. A., ... Lee, J. J. (2000). Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. Journal of Immunology, 165(10), 5509-5517. https://doi.org/10.4049/jimmunol.165.10.5509

Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. / Denzler, K. L.; Farmer, S. C.; Crosby, J. R.; Borchers, M.; Cieslewicz, G.; Larson, K. A.; Cormier, Stephania; Lee, N. A.; Lee, J. J.

In: Journal of Immunology, Vol. 165, No. 10, 15.11.2000, p. 5509-5517.

Research output: Contribution to journalArticle

Denzler, KL, Farmer, SC, Crosby, JR, Borchers, M, Cieslewicz, G, Larson, KA, Cormier, S, Lee, NA & Lee, JJ 2000, 'Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma', Journal of Immunology, vol. 165, no. 10, pp. 5509-5517. https://doi.org/10.4049/jimmunol.165.10.5509
Denzler, K. L. ; Farmer, S. C. ; Crosby, J. R. ; Borchers, M. ; Cieslewicz, G. ; Larson, K. A. ; Cormier, Stephania ; Lee, N. A. ; Lee, J. J. / Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. In: Journal of Immunology. 2000 ; Vol. 165, No. 10. pp. 5509-5517.
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