Epidermal growth factor (eof) suppresses oxidant-induced protein tyrosine phosphorylation (ftp) in caco-2 cell monolayers

R. K. Rao, S. S. Baker, R. D. Baker, M. Hpjycrpsjà

Research output: Contribution to journalArticle

Abstract

Oxidative stress is known to cause an increase in cellular PTP. Hydrogen peroxide (H.Q:) has been shown to increase tyrosine phosphorylation of a number of cellular proteins that may play a role in the oxidant-induced cell injury. In the present study, we analyzed the effect of H;O: on intestinal epithelial integrity and PTP using Caco-2 (colon adenocarcinoma) cells grown to umflucncy as monolaycrs on polycarbonate membranes (form a tight monolayer of epithelium). Additionally, we evaluated the effect of EOF on H 0.-induced PTP in these cells. PTP was analyzed by Western blot technique using anu-phosphotyrosine antibody, Exposure of Caco-2 cell monolaycrs to HO 110 mM) resulted in a decrease in transepithelial electrical resistance (TER), while pretreatment of monolayers with apical or basal EGF (30 nM) prevented this H-O-induccd decrease in TER. Exposure to FJ 0 increased tyrosine phosphorylation of a number of proteins (especially those with the M W range of 150-200. 110-130, 60-90. 54 & 44 kDa) in a dose- and timedependent manner. Pretreatment of monolayers with apical or basal EGF (1-30 nM) suppressed H;O -induced phosphorylation of these proteins in a dosedependent manner. We speculate that H:O; increases epithelial permeability by increasing PTP in Caco-2 cells, and that EGF protects epithelium from oxidantmiurv. which involves suppression of uxidant-induccd PTP.

Original languageEnglish (US)
Pages (from-to)A349
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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Phosphorylation
Caco-2 Cells
Epidermal Growth Factor
Oxidants
Tyrosine
Monolayers
polycarbonate
Acoustic impedance
Electric Impedance
Epithelium
Phosphotyrosine
Proteins
Oxidative stress
Hydrogen Peroxide
Permeability
Colon
Adenocarcinoma
Oxidative Stress
Western Blotting
Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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Epidermal growth factor (eof) suppresses oxidant-induced protein tyrosine phosphorylation (ftp) in caco-2 cell monolayers. / Rao, R. K.; Baker, S. S.; Baker, R. D.; Hpjycrpsjà, M.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996, p. A349.

Research output: Contribution to journalArticle

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