Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury

Radhakrishna Rao, Frank Porreca

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalEuropean Journal of Pharmacology
Volume303
Issue number3
DOIs
StatePublished - May 15 1996
Externally publishedYes

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Octoxynol
Epidermal Growth Factor
Mucous Membrane
Wounds and Injuries
Buffers

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury. / Rao, Radhakrishna; Porreca, Frank.

In: European Journal of Pharmacology, Vol. 303, No. 3, 15.05.1996, p. 209-212.

Research output: Contribution to journalArticle

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