Epitopes of group A streptococcal M protein shared with antigens of articular cartilage and synovium

R. W. Baird, M. S. Bronze, W. Kraus, H. R. Hill, L. G. Veasey, James Dale

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Abstract

Rabbit antisera evoked by purified pepsin-extracted group A streptococcal M proteins were screened for the presence of joint cross-reactive antibodies by indirect immunofluorescence using thin sections of mouse knee joints. Pep M1, M5, and M18 antisera contained antibodies that cross-reacted with chondrocytes, cartilage, and synovium. Immunoflourescence inhibition assays showed that some of the joint cross-reactive epitopes were shared among the three heterologous serotypes of M protein. The pep M5 joint cross-reactive epitopes were localized to three different synthetic peptides of the C-terminal region of pep M5. Immunoblot analyses showed that the M5 joint cross-reactive antibodies recognized two proteins of human synovium and cartilage of molecular mass 56 and 58 kDa. The cross-reactive antibodies binding to the 56-kDa protein were inhibited by purified vimentin in immunoblot inhibition experiments. M protein-specific antibodies from patients with acute rheumatic fever were also shown to cross-react with joint tissue in a pattern similar to the rabbit antisera. Rabbit and human M protein-specific antibodies that were bound to articular cartilage activated significant levels of complement when compared to control serum, suggesting that M protein joint cross-reactive antibodies could potentially be involved in the pathogenesis of ARF and arthritis.

Original languageEnglish (US)
Pages (from-to)3132-3137
Number of pages6
JournalJournal of Immunology
Volume146
Issue number9
StatePublished - Jan 1 1991

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Synovial Membrane
Articular Cartilage
Epitopes
Joints
Antigens
Antibodies
Immune Sera
Proteins
Rabbits
Cartilage
Rheumatic Fever
Pepsin A
Vimentin
Indirect Fluorescent Antibody Technique
Chondrocytes
Knee Joint
streptococcal M protein
Arthritis
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Baird, R. W., Bronze, M. S., Kraus, W., Hill, H. R., Veasey, L. G., & Dale, J. (1991). Epitopes of group A streptococcal M protein shared with antigens of articular cartilage and synovium. Journal of Immunology, 146(9), 3132-3137.

Epitopes of group A streptococcal M protein shared with antigens of articular cartilage and synovium. / Baird, R. W.; Bronze, M. S.; Kraus, W.; Hill, H. R.; Veasey, L. G.; Dale, James.

In: Journal of Immunology, Vol. 146, No. 9, 01.01.1991, p. 3132-3137.

Research output: Contribution to journalArticle

Baird, RW, Bronze, MS, Kraus, W, Hill, HR, Veasey, LG & Dale, J 1991, 'Epitopes of group A streptococcal M protein shared with antigens of articular cartilage and synovium', Journal of Immunology, vol. 146, no. 9, pp. 3132-3137.
Baird, R. W. ; Bronze, M. S. ; Kraus, W. ; Hill, H. R. ; Veasey, L. G. ; Dale, James. / Epitopes of group A streptococcal M protein shared with antigens of articular cartilage and synovium. In: Journal of Immunology. 1991 ; Vol. 146, No. 9. pp. 3132-3137.
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