Epitopes of group A streptococcal M protein that evoke cross-protective local immune responses

Michael S. Bronze, Harry S. Courtney, James Dale

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The present studies were undertaken to identify conserved epitopes of group A streptococcal M proteins that evoke cross-protective mucosal immune responses. Two synthetic peptides copying conserved regions of type 5 M protein, designated SM5(235-264)C and SM5(265-291)C, were covalently linked to carrier molecules and their immunogenicity was tested in laboratory animals. Rabbit antisera against both peptides cross-reacted with multiple serotypes of group A streptococci, indicating that the peptides contained broadly cross-reactive, surface exposed M protein epitopes. Serum antipeptide antibodies adsorbed to the surface of heterologous type 24 streptococci passively protected mice against intranasal challenge infections. Mice that were actively immunized intranasally with each synthetic peptide covalently linked to the B subunit of cholera toxin were protected against colonization and death after intranasal challenge infections with type 24 streptococci in the absence of serum opsonic antibodies. These data confirm and extend previous observations that conserved M protein epitopes evoke cross-protective local immunity and may serve as the basis for broadly cross-protective M protein vaccines.

Original languageEnglish (US)
Pages (from-to)888-893
Number of pages6
JournalJournal of Immunology
Volume148
Issue number3
StatePublished - Feb 1 1992

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Epitopes
Streptococcus
Peptides
Proteins
Mucosal Immunity
Antibodies
Cholera Toxin
Laboratory Animals
Infection
Serum
Immune Sera
Immunity
Vaccines
Rabbits
streptococcal M protein

All Science Journal Classification (ASJC) codes

  • Immunology

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Epitopes of group A streptococcal M protein that evoke cross-protective local immune responses. / Bronze, Michael S.; Courtney, Harry S.; Dale, James.

In: Journal of Immunology, Vol. 148, No. 3, 01.02.1992, p. 888-893.

Research output: Contribution to journalArticle

Bronze, Michael S. ; Courtney, Harry S. ; Dale, James. / Epitopes of group A streptococcal M protein that evoke cross-protective local immune responses. In: Journal of Immunology. 1992 ; Vol. 148, No. 3. pp. 888-893.
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