Epitopes of streptococcal M proteins that evoke antibodies that cross- react with human brain

M. S. Bronze, James Dale

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

There is evidence suggesting that Sydenham's chorea, which is a major manifestation of acute rheumatic fever, may be mediated by streptococcal antibodies that cross-react with the brain. Our studies were undertaken to determine whether streptococcal M protein, the major virulence factor of group A streptococci, evokes antibodies that cross-react with human brain. Rabbits were immunized with pepsin-extracted M protein from rheumatogenic type 6 streptococci. Immune sera were screened for the presence of antibodies that cross-reacted with human brain by indirect immunofluorescence tests and immunoblot analyses. Type 6 M protein evoked antibodies that cross-reacted with several brain proteins and antibody binding to these proteins was completely inhibited by type 6 M protein and partially inhibited by types 5 and 19 M proteins, suggesting that these heterologous M proteins contain conserved brain-cross-reactive epitopes. Using synthetic peptides from several serotypes of M proteins, the conserved brain-cross-reactive epitopes were localized to a decapeptide contained within the covalent structure of the B repeat region of type 6 M protein. These peptides also inhibited brain- cross-reactive antibodies in the serum of a patient with active Sydenham's chorea. Our data indicate that streptococcal M proteins contain brain-cross- reactive epitopes that could potentially be involved in the pathogenesis of Sydenham's chorea.

Original languageEnglish (US)
Pages (from-to)2820-2828
Number of pages9
JournalJournal of Immunology
Volume151
Issue number5
StatePublished - Jan 1 1993
Externally publishedYes

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Epitopes
Antibodies
Brain
Chorea
Proteins
Streptococcus
Peptides
Rheumatic Fever
streptococcal M protein
Pepsin A
Virulence Factors
Indirect Fluorescent Antibody Technique
Protein Binding
Immune Sera
Carrier Proteins
Rabbits
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Epitopes of streptococcal M proteins that evoke antibodies that cross- react with human brain. / Bronze, M. S.; Dale, James.

In: Journal of Immunology, Vol. 151, No. 5, 01.01.1993, p. 2820-2828.

Research output: Contribution to journalArticle

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