Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma

Margaret L. Gulley, Mark P. Burton, D. Craig Allred, John M. Nicholls, Mahul Amin, Jae Y. Ro, Barbara G. Schneider

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Eighty-three cases of nasopharyngeal carcinoma were evaluated for the presence of Epstein-Barr virus (EBV) infection in tumor cells by in situ hybridization to EBER1 transcripts, and for p53 expression by immunostains using the D07 antibody which detects native and mutant forms of the p53 protein. A highly significant association was found between EBV infection and p53 overexpression (P = .0004), with 77% of cases coexpressing both markers. This newly discovered association suggests that EBV is not an innocent bystander with respect to p53 accumulation. One possible mediator of the interaction between EBV and p53, viral BZLF1, was not colocalized with p53 in these tumors, suggesting that BZLF1 is not the factor responsible for p53 accumulation. From an epidemiological standpoint, this series of cancers represents an international cohort in which cases from an endemic part of the world (Hong Kong) were examined alongside cases from the United States, where the disease is 50-fold less prevalent. The cancers from Hong Kong tended to be less differentiated and more frequently associated with EBV, suggesting that biological differences might underlie epidemiological variations in tumor prevalence. Finally, we examined 18 potential premalignant lesions of the surface epithelium of the nasopharynx. Although our numbers are small, our data suggest that p53 accumulation might precede EBV infection in the transition from metaplasia to carcinoma in situ. Further studies are needed to dissect the stepwise progression of nopharyngeal carcinogenesis.

Original languageEnglish (US)
Pages (from-to)252-259
Number of pages8
JournalHuman pathology
Volume29
Issue number3
DOIs
StatePublished - Jan 1 1998

Fingerprint

Epstein-Barr Virus Infections
Human Herpesvirus 4
Hong Kong
Neoplasms
Nasopharynx
Carcinoma in Situ
Metaplasia
In Situ Hybridization
Carcinogenesis
Epithelium
Nasopharyngeal carcinoma
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Gulley, M. L., Burton, M. P., Allred, D. C., Nicholls, J. M., Amin, M., Ro, J. Y., & Schneider, B. G. (1998). Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma. Human pathology, 29(3), 252-259. https://doi.org/10.1016/S0046-8177(98)90044-2

Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma. / Gulley, Margaret L.; Burton, Mark P.; Allred, D. Craig; Nicholls, John M.; Amin, Mahul; Ro, Jae Y.; Schneider, Barbara G.

In: Human pathology, Vol. 29, No. 3, 01.01.1998, p. 252-259.

Research output: Contribution to journalArticle

Gulley, ML, Burton, MP, Allred, DC, Nicholls, JM, Amin, M, Ro, JY & Schneider, BG 1998, 'Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma', Human pathology, vol. 29, no. 3, pp. 252-259. https://doi.org/10.1016/S0046-8177(98)90044-2
Gulley, Margaret L. ; Burton, Mark P. ; Allred, D. Craig ; Nicholls, John M. ; Amin, Mahul ; Ro, Jae Y. ; Schneider, Barbara G. / Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma. In: Human pathology. 1998 ; Vol. 29, No. 3. pp. 252-259.
@article{4861edea2a064c7e9c0c5a32690aa00b,
title = "Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma",
abstract = "Eighty-three cases of nasopharyngeal carcinoma were evaluated for the presence of Epstein-Barr virus (EBV) infection in tumor cells by in situ hybridization to EBER1 transcripts, and for p53 expression by immunostains using the D07 antibody which detects native and mutant forms of the p53 protein. A highly significant association was found between EBV infection and p53 overexpression (P = .0004), with 77{\%} of cases coexpressing both markers. This newly discovered association suggests that EBV is not an innocent bystander with respect to p53 accumulation. One possible mediator of the interaction between EBV and p53, viral BZLF1, was not colocalized with p53 in these tumors, suggesting that BZLF1 is not the factor responsible for p53 accumulation. From an epidemiological standpoint, this series of cancers represents an international cohort in which cases from an endemic part of the world (Hong Kong) were examined alongside cases from the United States, where the disease is 50-fold less prevalent. The cancers from Hong Kong tended to be less differentiated and more frequently associated with EBV, suggesting that biological differences might underlie epidemiological variations in tumor prevalence. Finally, we examined 18 potential premalignant lesions of the surface epithelium of the nasopharynx. Although our numbers are small, our data suggest that p53 accumulation might precede EBV infection in the transition from metaplasia to carcinoma in situ. Further studies are needed to dissect the stepwise progression of nopharyngeal carcinogenesis.",
author = "Gulley, {Margaret L.} and Burton, {Mark P.} and Allred, {D. Craig} and Nicholls, {John M.} and Mahul Amin and Ro, {Jae Y.} and Schneider, {Barbara G.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1016/S0046-8177(98)90044-2",
language = "English (US)",
volume = "29",
pages = "252--259",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma

AU - Gulley, Margaret L.

AU - Burton, Mark P.

AU - Allred, D. Craig

AU - Nicholls, John M.

AU - Amin, Mahul

AU - Ro, Jae Y.

AU - Schneider, Barbara G.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Eighty-three cases of nasopharyngeal carcinoma were evaluated for the presence of Epstein-Barr virus (EBV) infection in tumor cells by in situ hybridization to EBER1 transcripts, and for p53 expression by immunostains using the D07 antibody which detects native and mutant forms of the p53 protein. A highly significant association was found between EBV infection and p53 overexpression (P = .0004), with 77% of cases coexpressing both markers. This newly discovered association suggests that EBV is not an innocent bystander with respect to p53 accumulation. One possible mediator of the interaction between EBV and p53, viral BZLF1, was not colocalized with p53 in these tumors, suggesting that BZLF1 is not the factor responsible for p53 accumulation. From an epidemiological standpoint, this series of cancers represents an international cohort in which cases from an endemic part of the world (Hong Kong) were examined alongside cases from the United States, where the disease is 50-fold less prevalent. The cancers from Hong Kong tended to be less differentiated and more frequently associated with EBV, suggesting that biological differences might underlie epidemiological variations in tumor prevalence. Finally, we examined 18 potential premalignant lesions of the surface epithelium of the nasopharynx. Although our numbers are small, our data suggest that p53 accumulation might precede EBV infection in the transition from metaplasia to carcinoma in situ. Further studies are needed to dissect the stepwise progression of nopharyngeal carcinogenesis.

AB - Eighty-three cases of nasopharyngeal carcinoma were evaluated for the presence of Epstein-Barr virus (EBV) infection in tumor cells by in situ hybridization to EBER1 transcripts, and for p53 expression by immunostains using the D07 antibody which detects native and mutant forms of the p53 protein. A highly significant association was found between EBV infection and p53 overexpression (P = .0004), with 77% of cases coexpressing both markers. This newly discovered association suggests that EBV is not an innocent bystander with respect to p53 accumulation. One possible mediator of the interaction between EBV and p53, viral BZLF1, was not colocalized with p53 in these tumors, suggesting that BZLF1 is not the factor responsible for p53 accumulation. From an epidemiological standpoint, this series of cancers represents an international cohort in which cases from an endemic part of the world (Hong Kong) were examined alongside cases from the United States, where the disease is 50-fold less prevalent. The cancers from Hong Kong tended to be less differentiated and more frequently associated with EBV, suggesting that biological differences might underlie epidemiological variations in tumor prevalence. Finally, we examined 18 potential premalignant lesions of the surface epithelium of the nasopharynx. Although our numbers are small, our data suggest that p53 accumulation might precede EBV infection in the transition from metaplasia to carcinoma in situ. Further studies are needed to dissect the stepwise progression of nopharyngeal carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0031939038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031939038&partnerID=8YFLogxK

U2 - 10.1016/S0046-8177(98)90044-2

DO - 10.1016/S0046-8177(98)90044-2

M3 - Article

VL - 29

SP - 252

EP - 259

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 3

ER -