Equine light-chain-associated amyloidosis

Theodoor A. Niewold, Charles Murphy, Erik Gruys, Deborah T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have previously immunologically typed amyloid protein extracted from a horse with malignant histiolymphocytic lymphosarcoma as immunoglobulin (Ig)-derived amyloid. In the present paper, the Ig character of the horse amyloid is confirmed by the amino acid sequence of the constituent protein. Its major component is identified as a 217-residue complete horse λ light chain. On the basis of sequence homology, the variable (V) and joining (J) segments were most closely related to those encoded by the equine Vλ2- and Jλ1-gene families, respectively. Notably, the constant (C) region, although most closely related to the Cλ1-gene, differed from the prototypic sequence by five residues. These findings have provided further evidence that proteolytic precleavage of the precursor is not essential in light-chain-associated (AL) amyloidogenesis. In addition to establishing the first complete amino acid sequence of a horse Ig light chain, the discovery of a unique C-regionprimary structure implies extensive Cλ1 polymorphism or the existence of a heretofore unrecognized equine Cλ gene.

Original languageEnglish (US)
Pages (from-to)183-186
Number of pages4
JournalAmyloid
Volume3
Issue number3
DOIs
StatePublished - Jan 1 1996

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Amyloidosis
Horses
Light
Amyloid
Immunoglobulins
Amino Acid Sequence
Genes
Immunoglobulin Light Chains
Amyloidogenic Proteins
Sequence Homology
Non-Hodgkin's Lymphoma
Proteins

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Niewold, T. A., Murphy, C., Gruys, E., Weiss, D. T., & Solomon, A. (1996). Equine light-chain-associated amyloidosis. Amyloid, 3(3), 183-186. https://doi.org/10.3109/13506129609045520

Equine light-chain-associated amyloidosis. / Niewold, Theodoor A.; Murphy, Charles; Gruys, Erik; Weiss, Deborah T.; Solomon, Alan.

In: Amyloid, Vol. 3, No. 3, 01.01.1996, p. 183-186.

Research output: Contribution to journalArticle

Niewold, TA, Murphy, C, Gruys, E, Weiss, DT & Solomon, A 1996, 'Equine light-chain-associated amyloidosis', Amyloid, vol. 3, no. 3, pp. 183-186. https://doi.org/10.3109/13506129609045520
Niewold TA, Murphy C, Gruys E, Weiss DT, Solomon A. Equine light-chain-associated amyloidosis. Amyloid. 1996 Jan 1;3(3):183-186. https://doi.org/10.3109/13506129609045520
Niewold, Theodoor A. ; Murphy, Charles ; Gruys, Erik ; Weiss, Deborah T. ; Solomon, Alan. / Equine light-chain-associated amyloidosis. In: Amyloid. 1996 ; Vol. 3, No. 3. pp. 183-186.
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