ERα regulates lipid metabolism in bone through ATGL and perilipin

Korinna Wend, Peter Wend, Brian G. Drew, Andrea L. Hevener, Gustavo Miranda-Carboni, Susan Miranda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A decrease in bone mineral density during menopause is accompanied by an increase in adipocytes in the bone marrow space. Ovariectomy also leads to accumulation of fat in the bone marrow. Herein we show increased lipid accumulation in bone marrow from estrogen receptor alpha (ERα) knockout (ERαKO) mice compared to wild-type (WT) mice or estrogen receptor beta (ERβ) knockout (ERβKO) mice. Similarly, bone marrow cells from ERαKO mice differentiated to adipocytes in culture also have increased lipid accumulation compared to cells from WT mice or ERβKO mice. Analysis of individual adipocytes shows that WT mice have fewer, but larger, lipid droplets per cell than adipocytes from ERαKO or ERβKO animals. Furthermore, higher levels of adipose triglyceride lipase (ATGL) protein in WT adipocytes correlate with increased lipolysis and fewer lipid droplets per cell and treatment with 17β-estradiol (E2) potentiates this response. In contrast, cells from ERαKO mice display higher perilipin protein levels, promoting lipogenesis. Together these results demonstrate that E2 signals via ERα to regulate lipid droplet size and total lipid accumulation in the bone marrow space in vivo. J. Cell. Biochem. 114: 1306-1314, 2013. © 2012 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)1306-1314
Number of pages9
JournalJournal of Cellular Biochemistry
Volume114
Issue number6
DOIs
StatePublished - Jun 1 2013

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Estrogen Receptor alpha
Lipase
Lipid Metabolism
Adipocytes
Knockout Mice
Bone
Lipids
Bone and Bones
Bone Marrow
Estrogen Receptor beta
Lipogenesis
Lipolysis
Ovariectomy
Menopause
Bone Marrow Cells
Bone Density
Estradiol
Cell culture
Fats
Minerals

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

ERα regulates lipid metabolism in bone through ATGL and perilipin. / Wend, Korinna; Wend, Peter; Drew, Brian G.; Hevener, Andrea L.; Miranda-Carboni, Gustavo; Miranda, Susan.

In: Journal of Cellular Biochemistry, Vol. 114, No. 6, 01.06.2013, p. 1306-1314.

Research output: Contribution to journalArticle

Wend, Korinna ; Wend, Peter ; Drew, Brian G. ; Hevener, Andrea L. ; Miranda-Carboni, Gustavo ; Miranda, Susan. / ERα regulates lipid metabolism in bone through ATGL and perilipin. In: Journal of Cellular Biochemistry. 2013 ; Vol. 114, No. 6. pp. 1306-1314.
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