Erythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy

G. Bhanuprakash Reddy, Satyanarayana Alleboina, N. Balakrishna, Radha Ayyagari, M. Padma, K. Viswanath, J. Mark Petrash

Research output: Contribution to journalArticle

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Abstract

Purpose: Activation of polyol pathway due to increased aldose reductase (ALR2) activity has been implicated in the development of diabetic complications including diabetic retinopathy (DR), a leading cause of blindness. However, the relationship between hyperglycemia-induced activation of polyol pathway in retina and DR is still uncertain. We investigated the relationship between ALR2 levels and human DR by measuring ALR2 activity and its product, sorbitol, in erythrocytes. Methods: We enrolled 362 type 2 diabetic subjects (T2D) with and without DR and 66 normal subjects in this clinical case-control study. Clinical evaluation of DR in T2D patients was done by fundus examination. ALR2 activity and sorbitol levels along with glucose and glycosylated hemoglobin (HbA1C) levels in erythrocytes were determined. Results: T2D patients with DR showed significantly higher specific activity of ALR2 as compared to T2D patients without DR. Elevated levels of sorbitol in T2D patients with DR, as compared to T2D patients without DR, corroborated the increased ALR2 activity in erythrocytes of DR patients. However, the increased ALR2 activity was not significantly associated with diabetes duration, age, and HbA1C in both the DR group and total T2D subjects. Conclusions: Levels of ALR2 activity as well as sorbitol in erythrocytes may have value as a quantitative trait to be included among other markers to establish a risk profile for development of DR.

Original languageEnglish (US)
Pages (from-to)593-601
Number of pages9
JournalMolecular vision
Volume14
StatePublished - Mar 24 2008

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Aldehyde Reductase
Sorbitol
Diabetic Retinopathy
Erythrocytes
Glycosylated Hemoglobin A
Diabetes Complications
Blindness
Hyperglycemia
Retina
Case-Control Studies

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Reddy, G. B., Alleboina, S., Balakrishna, N., Ayyagari, R., Padma, M., Viswanath, K., & Petrash, J. M. (2008). Erythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy. Molecular vision, 14, 593-601.

Erythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy. / Reddy, G. Bhanuprakash; Alleboina, Satyanarayana; Balakrishna, N.; Ayyagari, Radha; Padma, M.; Viswanath, K.; Petrash, J. Mark.

In: Molecular vision, Vol. 14, 24.03.2008, p. 593-601.

Research output: Contribution to journalArticle

Reddy, GB, Alleboina, S, Balakrishna, N, Ayyagari, R, Padma, M, Viswanath, K & Petrash, JM 2008, 'Erythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy', Molecular vision, vol. 14, pp. 593-601.
Reddy GB, Alleboina S, Balakrishna N, Ayyagari R, Padma M, Viswanath K et al. Erythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy. Molecular vision. 2008 Mar 24;14:593-601.
Reddy, G. Bhanuprakash ; Alleboina, Satyanarayana ; Balakrishna, N. ; Ayyagari, Radha ; Padma, M. ; Viswanath, K. ; Petrash, J. Mark. / Erythrocyte aldose reductase activity and sorbitol levels in diabetic retinopathy. In: Molecular vision. 2008 ; Vol. 14. pp. 593-601.
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