Erythrocyte binding properties of streptococcal lipoteichoic acids

E. H. Beachey, James Dale, W. A. Simpson, J. D. Evans, K. W. Knox, I. Ofek, A. J. Wicken

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The lipoteichoic acids (LTA) of gram-positive bacteria are known to bind spontaneously to a variety of animal cell membranes. The authors investigated the biological and biochemical characteristics of the binding of LTA of Streptococcus pyogenes and S. faecalis to human and sheep erythrocytes. The kinetics of the binding of the radiolabeled LTA ([ 3H]LTA) from each of these organisms to erythrocytes was similar. The dissociation constants for sheep and adult human erythrocytes were 1.6 μM and 4.5μM, respectively, whereas that of human cord blood erythrocytes was approximately 10-fold higher, 31 μM. The number of binding sites for sheep erythrocytes was calculated to be 7.2 x x10 6 per cell, and that of human erythrocytes, 29 x 10 6 per cell. Binding was reversible. More than 50% of bound [ 3H]LTA was displaced from erythrocytes by a 50-fold excess of unlabeled LTA. LTA prepared from heterologous species of gram-positive bacteria were all inhibitory to the binding of [ 3H]LTA whether derived from S. pyogenes or from S. faecalis. Among a number of potential receptor analogues and other inhibitors tested, including serum albumin, gangliosides Gm 2 and Gm 3, lipopolysaccharide of gram-negative bacteria, and various sugars, only albumin and the gangliosides significantly inhibited LTA binding. Trypsin or neuraminidase treatment of erythrocytes had no effect on LTA binding. Deacylation of [ 3H]LTA abolished binding ability and binding was restored by esterification of the deacylated material with stearoyl chloride, indicating that ester-linked lipids are necessary for membrane binding.

Original languageEnglish (US)
Pages (from-to)618-625
Number of pages8
JournalInfection and Immunity
Volume23
Issue number3
StatePublished - Oct 19 1979
Externally publishedYes

Fingerprint

Erythrocytes
Sheep
Gangliosides
Streptococcus pyogenes
Gram-Positive Bacteria
lipoteichoic acid
Esterification
Neuraminidase
Membrane Lipids
Gram-Negative Bacteria
Fetal Blood
Serum Albumin
Trypsin
Lipopolysaccharides
Albumins
Esters
Binding Sites
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Beachey, E. H., Dale, J., Simpson, W. A., Evans, J. D., Knox, K. W., Ofek, I., & Wicken, A. J. (1979). Erythrocyte binding properties of streptococcal lipoteichoic acids. Infection and Immunity, 23(3), 618-625.

Erythrocyte binding properties of streptococcal lipoteichoic acids. / Beachey, E. H.; Dale, James; Simpson, W. A.; Evans, J. D.; Knox, K. W.; Ofek, I.; Wicken, A. J.

In: Infection and Immunity, Vol. 23, No. 3, 19.10.1979, p. 618-625.

Research output: Contribution to journalArticle

Beachey, EH, Dale, J, Simpson, WA, Evans, JD, Knox, KW, Ofek, I & Wicken, AJ 1979, 'Erythrocyte binding properties of streptococcal lipoteichoic acids', Infection and Immunity, vol. 23, no. 3, pp. 618-625.
Beachey EH, Dale J, Simpson WA, Evans JD, Knox KW, Ofek I et al. Erythrocyte binding properties of streptococcal lipoteichoic acids. Infection and Immunity. 1979 Oct 19;23(3):618-625.
Beachey, E. H. ; Dale, James ; Simpson, W. A. ; Evans, J. D. ; Knox, K. W. ; Ofek, I. ; Wicken, A. J. / Erythrocyte binding properties of streptococcal lipoteichoic acids. In: Infection and Immunity. 1979 ; Vol. 23, No. 3. pp. 618-625.
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