Erythrocyte membrane skeletal protein bands 4.1 a and b are sequence-related phosphoproteins.

Steven Goodman, J. Yu, C. F. Whitfield, E. N. Culp, E. J. Posnak

Research output: Contribution to journalArticle

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Abstract

Bands 4.1 a and b are proteins of 80,000 and 78,000 molecular weight, which are both present at approximately 100,000 copies per erythrocyte ghost. Both proteins are components of the erythrocyte membrane skeleton. Bands 4.1 a and b are labeled when intact erythrocytes are incubated with [32P]orthophosphoric acid, and, therefore, are phosphoproteins. One-dimensional partial proteolytic mapping analysis of 32P-labeled bands 4.1 a and 4.1 b and two-dimensional peptide mapping analysis of 125I-labeled bands 4.1 a and 4.1 b clearly demonstrated that the two proteins are sequence-related phosphoproteins. Band 4.1 purified by standard techniques (Tyler, J. M., Hargreaves, W. R., and Branton, D. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 5192-5196) contains bands 4.1 a and 4.1 b. Bands 4.1 a and 4.1 b bind to spectrin heterodimers in solution. We conclude that the erythrocyte skeletal proteins bands 4.1 a and 4.1 b are sequence-related phosphoproteins, both capable of binding spectrin.

Original languageEnglish (US)
Pages (from-to)4564-4569
Number of pages6
JournalJournal of Biological Chemistry
Volume257
Issue number8
StatePublished - Apr 25 1982
Externally publishedYes

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Phosphoproteins
Erythrocyte Membrane
Spectrin
Membranes
Erythrocytes
Proteins
Peptide Mapping
Skeleton
Molecular Weight
Molecular weight
Peptides
erythrocyte membrane band 4.1 protein

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Erythrocyte membrane skeletal protein bands 4.1 a and b are sequence-related phosphoproteins. / Goodman, Steven; Yu, J.; Whitfield, C. F.; Culp, E. N.; Posnak, E. J.

In: Journal of Biological Chemistry, Vol. 257, No. 8, 25.04.1982, p. 4564-4569.

Research output: Contribution to journalArticle

Goodman, Steven ; Yu, J. ; Whitfield, C. F. ; Culp, E. N. ; Posnak, E. J. / Erythrocyte membrane skeletal protein bands 4.1 a and b are sequence-related phosphoproteins. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 8. pp. 4564-4569.
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AU - Posnak, E. J.

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N2 - Bands 4.1 a and b are proteins of 80,000 and 78,000 molecular weight, which are both present at approximately 100,000 copies per erythrocyte ghost. Both proteins are components of the erythrocyte membrane skeleton. Bands 4.1 a and b are labeled when intact erythrocytes are incubated with [32P]orthophosphoric acid, and, therefore, are phosphoproteins. One-dimensional partial proteolytic mapping analysis of 32P-labeled bands 4.1 a and 4.1 b and two-dimensional peptide mapping analysis of 125I-labeled bands 4.1 a and 4.1 b clearly demonstrated that the two proteins are sequence-related phosphoproteins. Band 4.1 purified by standard techniques (Tyler, J. M., Hargreaves, W. R., and Branton, D. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 5192-5196) contains bands 4.1 a and 4.1 b. Bands 4.1 a and 4.1 b bind to spectrin heterodimers in solution. We conclude that the erythrocyte skeletal proteins bands 4.1 a and 4.1 b are sequence-related phosphoproteins, both capable of binding spectrin.

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