Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme

D. G. Kakhniashvili, T. Chaudhary, W. E. Zimmer, F. Aladar Bencsath, I. Jardine, Steven Goodman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The involvement of red blood cell spectrin in the ubiquitination process was studied. Spectrin was found to form two ubiquitin-associated derivatives, a DTT-sensitive ubiquitin adduct and a DTT-insensitive conjugate, characteristic intermediate and final products of the ubiquitination reaction cascade. In addition to spectrin and ubiquitin, ubiquitin-activating enzyme (E1) and ATP were necessary and sufficient to form both the spectrin-ubiquitin adduct and conjugate. No exogenous ubiquitin-conjugating (E2) or ligase (E3) activities were required, suggesting that erythrocyte spectrin is an E2 ubiquitin-conjugating enzyme able to target itself. Both ubiquitin adduct and conjugate were linked to the α subunit of spectrin, suggesting that the ubiquitin- conjugating (UBC) domain and its target regions reside on the same subunit.

Original languageEnglish (US)
Pages (from-to)11630-11642
Number of pages13
JournalBiochemistry
Volume40
Issue number38
DOIs
StatePublished - Sep 25 2001
Externally publishedYes

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Ubiquitin-Conjugating Enzymes
Spectrin
Ubiquitin
Erythrocytes
Ubiquitination
Ubiquitin-Activating Enzymes
Ubiquitin-Protein Ligases
Blood
Adenosine Triphosphate
Cells
Derivatives

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Kakhniashvili, D. G., Chaudhary, T., Zimmer, W. E., Aladar Bencsath, F., Jardine, I., & Goodman, S. (2001). Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme. Biochemistry, 40(38), 11630-11642. https://doi.org/10.1021/bi010176t

Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme. / Kakhniashvili, D. G.; Chaudhary, T.; Zimmer, W. E.; Aladar Bencsath, F.; Jardine, I.; Goodman, Steven.

In: Biochemistry, Vol. 40, No. 38, 25.09.2001, p. 11630-11642.

Research output: Contribution to journalArticle

Kakhniashvili, DG, Chaudhary, T, Zimmer, WE, Aladar Bencsath, F, Jardine, I & Goodman, S 2001, 'Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme', Biochemistry, vol. 40, no. 38, pp. 11630-11642. https://doi.org/10.1021/bi010176t
Kakhniashvili DG, Chaudhary T, Zimmer WE, Aladar Bencsath F, Jardine I, Goodman S. Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme. Biochemistry. 2001 Sep 25;40(38):11630-11642. https://doi.org/10.1021/bi010176t
Kakhniashvili, D. G. ; Chaudhary, T. ; Zimmer, W. E. ; Aladar Bencsath, F. ; Jardine, I. ; Goodman, Steven. / Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme. In: Biochemistry. 2001 ; Vol. 40, No. 38. pp. 11630-11642.
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