Estimating the proliferative activity in breast cancer

Is Ki-67 immunostaining necessary?

Mamoun Younes, Dina R. Mody, Charles C. Miller, Lia V. Lechago, Rodolfo Laucirica

Research output: Contribution to journalArticle

Abstract

The proliferative,activity (PA) of tumor cells has been shown to be a significant prognostic indicator in breast cancer. Ki-67 immunoreactivity in paraffin-embedded tissue has been shown to correlate with S-phase fraction as determined by flow cytometry. The aim of this work is to determine whether PA by Ki-67 immunostaining has significant advantage over mitotic figure count per ten high power fields (MF/1OHPF) or mitotic index (MI). Sections of formalin-fixed paraffin-embedded tissue of invasive breast cancer from 118 women were evaluated for MF/10HPF, MI, and immunostained for Ki-67 using the monoclonal antibody MIB-1. We found that PA determined by any of the three methods was a significant predictor of survival with p values of 0.016, 0.008, and 0.049 for PA determined by MI, MF/1OHPF, and Ki-67 respectively. We conclude that determining PA by KI-67 immunostaining does not have advantage over the traditional methods.

Original languageEnglish (US)
Pages (from-to)269-275
Number of pages7
JournalBreast Disease
Volume9
Issue number5
StatePublished - Sep 1 1996
Externally publishedYes

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Mitotic Index
Breast Neoplasms
Paraffin
S Phase
Formaldehyde
Flow Cytometry
Survival
Neoplasms

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Younes, M., Mody, D. R., Miller, C. C., Lechago, L. V., & Laucirica, R. (1996). Estimating the proliferative activity in breast cancer: Is Ki-67 immunostaining necessary? Breast Disease, 9(5), 269-275.

Estimating the proliferative activity in breast cancer : Is Ki-67 immunostaining necessary? / Younes, Mamoun; Mody, Dina R.; Miller, Charles C.; Lechago, Lia V.; Laucirica, Rodolfo.

In: Breast Disease, Vol. 9, No. 5, 01.09.1996, p. 269-275.

Research output: Contribution to journalArticle

Younes, M, Mody, DR, Miller, CC, Lechago, LV & Laucirica, R 1996, 'Estimating the proliferative activity in breast cancer: Is Ki-67 immunostaining necessary?', Breast Disease, vol. 9, no. 5, pp. 269-275.
Younes, Mamoun ; Mody, Dina R. ; Miller, Charles C. ; Lechago, Lia V. ; Laucirica, Rodolfo. / Estimating the proliferative activity in breast cancer : Is Ki-67 immunostaining necessary?. In: Breast Disease. 1996 ; Vol. 9, No. 5. pp. 269-275.
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