Eugenol dilates rat cerebral arteries by inhibiting smooth muscle cell voltage-dependent calcium channels

Dieniffer Peixoto-Neves, Jose Henrique Leal-Cardoso, Jonathan Jaggar

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Plants high in eugenol, a phenylpropanoid compound, are used as folk medicines to alleviate diseases including hypertension. Eugenol has been demonstrated to relax conduit and ear arteries and reduce systemic blood pressure, but mechanisms involved are unclear. Here, we studied eugenol regulation of resistance-size cerebral arteries that control regional brain blood pressure and flow and investigated mechanisms involved. We demonstrate that eugenol dilates arteries constricted by either pressure or membrane depolarization (60 mM K+) in a concentration-dependent manner. Experiments performed using patch-clamp electrophysiology demonstrated that eugenol inhibited voltage-dependent calcium (Ca2+) currents, when using Ba2+ as a charge carrier, in isolated cerebral artery smooth muscle cells. Eugenol inhibition of voltage-dependent Ca2+ currents involved pore block, a hyperpolarizing shift (∼-10 mV) in voltage-dependent inactivation, an increase in the proportion of steady-state inactivating current, and acceleration of inactivation rate. In summary, our data indicate that eugenol dilates cerebral arteries by means of multimodal inhibition of voltage-dependent Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)401-406
Number of pages6
JournalJournal of Cardiovascular Pharmacology
Volume64
Issue number5
DOIs
StatePublished - Nov 1 2014

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Eugenol
Cerebral Arteries
Calcium Channels
Smooth Muscle Myocytes
Arteries
Blood Pressure
Electrophysiology
Traditional Medicine
Ear
Hypertension
Calcium
Pressure
Membranes
Brain

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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Eugenol dilates rat cerebral arteries by inhibiting smooth muscle cell voltage-dependent calcium channels. / Peixoto-Neves, Dieniffer; Leal-Cardoso, Jose Henrique; Jaggar, Jonathan.

In: Journal of Cardiovascular Pharmacology, Vol. 64, No. 5, 01.11.2014, p. 401-406.

Research output: Contribution to journalArticle

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