Evaluation of a high-intensity focused ultrasound-immobilized trypsin digestion and 18O-labeling method for quantitative proteomics

Daniel Ló Pez-Ferrer, Kim K. Hixson, Heather Smallwood, Thomas C. Squier, Konstantinos Petritis, Richard D. Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A new method that uses immobilized trypsin concomitant with ultrasonic irradiation results in ultrarapid digestion and more thorough 18O labeling for quantitative protein comparisons. The method was reproducible and provided effective digestions within <1 min with lower amounts of enzyme, compared to traditional methods. This method was demonstrated for digestion of both simple and complex protein mixtures, including bovine serum albumin, a global proteome extract from the bacteria Shewanella oneidensis, and mouse plasma, as well as 18O labeling of complex protein mixtures, validating this method for differential proteomic measurements. This approach is simple, reproducible, cost-effective, rapid, and well suited for automation.

Original languageEnglish (US)
Pages (from-to)6272-6277
Number of pages6
JournalAnalytical Chemistry
Volume81
Issue number15
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

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Trypsin
Labeling
Ultrasonics
Proteins
Proteome
Bovine Serum Albumin
Bacteria
Automation
Irradiation
Plasmas
Enzymes
Proteomics
Costs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Evaluation of a high-intensity focused ultrasound-immobilized trypsin digestion and 18O-labeling method for quantitative proteomics. / Pez-Ferrer, Daniel Ló; Hixson, Kim K.; Smallwood, Heather; Squier, Thomas C.; Petritis, Konstantinos; Smith, Richard D.

In: Analytical Chemistry, Vol. 81, No. 15, 01.08.2009, p. 6272-6277.

Research output: Contribution to journalArticle

Pez-Ferrer, Daniel Ló ; Hixson, Kim K. ; Smallwood, Heather ; Squier, Thomas C. ; Petritis, Konstantinos ; Smith, Richard D. / Evaluation of a high-intensity focused ultrasound-immobilized trypsin digestion and 18O-labeling method for quantitative proteomics. In: Analytical Chemistry. 2009 ; Vol. 81, No. 15. pp. 6272-6277.
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