Evaluation of amniotic multipotential tissue matrix to augment healing of demineralized bone matrix in an animal calvarial model

Petros Konofaos, Dana Petersen, Jessica A. Jennings, Richard Smith, Heather Doty, Benjamin T. Reves, Teja Guda, Mark R. Appleford, Joel D. Bumgardner, Robert Wallace

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Amniotic multipotential tissue matrix (AmnioMTM) is a membrane material derived from placental tissues and rich in growth factors that have been reported to have potential in healing bone. This study hypothesized that demineralized bone matrix (DBM) supplemented with AmnioMTM would accelerate healing and bone formation as compared with DBM alone in a critical size (10 mm) rat calvarial bone defect model. Five DBM grafts and 5 DBM supplemented with AmnioMTM grafts were implanted in a 10-mm critical sized defect in 10 rats (1 implant per rat). After 4 weeks, animals were euthanized and defects evaluated by microCT and histology. There were no statistical differences in microCT data for mineral density, percent bone fill, or bone surface to volume ratios between groups, though the bone surface to volume ratio for the amnio-supplemented group suggested increased osteoid activity as compared with the DBM alone group. Histological data also indicated active osteoid activity and induced bone formation in the center of defects implanted with AmnioMTM supplemented graft as compared with DBM graft alone suggesting some potential osteoinductive potential. However, there was no significant difference at the mean percent of newly mineralized bone in the DBM group defect as compared with the AmnioMTM supplemented graft material. These data suggest that while bone formation was not increased at this early time point, the increased osteoid activity and the induction of new bone in the middle of the defect by the AmnioMTM indicates that further study is needed to assess its potential benefit to bone healing and regeneration.

Original languageEnglish (US)
Pages (from-to)1408-1412
Number of pages5
JournalJournal of Craniofacial Surgery
Volume26
Issue number4
DOIs
StatePublished - Jun 1 2015

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Bone Matrix
Animal Models
Bone and Bones
Transplants
Osteogenesis
X-Ray Microtomography
Bone Regeneration
Bone Density
Intercellular Signaling Peptides and Proteins
Histology
Membranes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Otorhinolaryngology

Cite this

Evaluation of amniotic multipotential tissue matrix to augment healing of demineralized bone matrix in an animal calvarial model. / Konofaos, Petros; Petersen, Dana; Jennings, Jessica A.; Smith, Richard; Doty, Heather; Reves, Benjamin T.; Guda, Teja; Appleford, Mark R.; Bumgardner, Joel D.; Wallace, Robert.

In: Journal of Craniofacial Surgery, Vol. 26, No. 4, 01.06.2015, p. 1408-1412.

Research output: Contribution to journalArticle

Konofaos, Petros ; Petersen, Dana ; Jennings, Jessica A. ; Smith, Richard ; Doty, Heather ; Reves, Benjamin T. ; Guda, Teja ; Appleford, Mark R. ; Bumgardner, Joel D. ; Wallace, Robert. / Evaluation of amniotic multipotential tissue matrix to augment healing of demineralized bone matrix in an animal calvarial model. In: Journal of Craniofacial Surgery. 2015 ; Vol. 26, No. 4. pp. 1408-1412.
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