Evaluation of gene panel mRNAs in urine samples of kidney transplant recipients as a non-invasive tool of graft function

Valeria Mas, Luciana A. Mas, Kellie J. Archer, Kenneth Yanek, Anne L. King, Eric M. Gibney, Adrian Cotterell, Robert A. Fisher, Marc Posner, Daniel Maluf

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Non-invasive monitoring may be useful after kidney transplantation (KT), particularly for predicting acute rejection (AR). It is less clear whether chronic allograft nephropathy (CAN) is also associated with changes in urine cells. To identify non-invasive markers of allograft function in kidney transplant patients (KTP), mRNA levels of AGT, TGF-β1, EGFR, IFN-γ, TSP-1, and IL-10 in urine (Ur) samples were studied using QRT-PCR. Ninety-five KTP and 111 Ur samples were evaluated. Patients (Pts) were divided as, within six months (N = 31), and with more than six months post-KT (N = 64), KTP with more than six months post-KT were classified as KTP with stable kidney function (SKF) (N = 32), KTP with SKF (creatinine < 2 mg/dL) and proteinuria > 500 mg/24 h (N = 18), and KTP with biopsy proven CAN (N = 14). F-test was used to test for equality of variances between groups. IL-10 mRNA was decreased in Ur samples from KTP with less than six months post-KT (P = 0.005). For KTR groups with more than six months post-KT, AGT and EGFR mRNA were statistically different among KTP with SKF, KTP with SKF and proteinuria, and CAN Pts (P = 0.003, and P = 0.01), with KTP with SKF having higher mean expression. TSP-1 mRNA levels also were significantly different among these three groups (P = 0.04), with higher expression observed in CAN Pts. Using the random forest algorithm, AGT, EGFR, and TGF-β1 were identified as predictors of CAN, SKF, SKF with proteinuria. A characteristic pattern of mRNA levels in the different KTP groups was observed indicating that the mRNA levels in Ur cells might reflect allograft function.

Original languageEnglish (US)
Pages (from-to)315-324
Number of pages10
JournalMolecular Medicine
Volume13
Issue number5-6
DOIs
StatePublished - May 1 2007

Fingerprint

Urine
Transplants
Kidney
Messenger RNA
Genes
Allografts
Kidney Transplantation
Thrombospondin 1
Transplant Recipients
Proteinuria
Interleukin-10
Creatinine
Biopsy

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Evaluation of gene panel mRNAs in urine samples of kidney transplant recipients as a non-invasive tool of graft function. / Mas, Valeria; Mas, Luciana A.; Archer, Kellie J.; Yanek, Kenneth; King, Anne L.; Gibney, Eric M.; Cotterell, Adrian; Fisher, Robert A.; Posner, Marc; Maluf, Daniel.

In: Molecular Medicine, Vol. 13, No. 5-6, 01.05.2007, p. 315-324.

Research output: Contribution to journalArticle

Mas, V, Mas, LA, Archer, KJ, Yanek, K, King, AL, Gibney, EM, Cotterell, A, Fisher, RA, Posner, M & Maluf, D 2007, 'Evaluation of gene panel mRNAs in urine samples of kidney transplant recipients as a non-invasive tool of graft function', Molecular Medicine, vol. 13, no. 5-6, pp. 315-324. https://doi.org/10.2119/2007-00017.Mas
Mas, Valeria ; Mas, Luciana A. ; Archer, Kellie J. ; Yanek, Kenneth ; King, Anne L. ; Gibney, Eric M. ; Cotterell, Adrian ; Fisher, Robert A. ; Posner, Marc ; Maluf, Daniel. / Evaluation of gene panel mRNAs in urine samples of kidney transplant recipients as a non-invasive tool of graft function. In: Molecular Medicine. 2007 ; Vol. 13, No. 5-6. pp. 315-324.
@article{b3f498e426bb4f03852353b5ff765e15,
title = "Evaluation of gene panel mRNAs in urine samples of kidney transplant recipients as a non-invasive tool of graft function",
abstract = "Non-invasive monitoring may be useful after kidney transplantation (KT), particularly for predicting acute rejection (AR). It is less clear whether chronic allograft nephropathy (CAN) is also associated with changes in urine cells. To identify non-invasive markers of allograft function in kidney transplant patients (KTP), mRNA levels of AGT, TGF-β1, EGFR, IFN-γ, TSP-1, and IL-10 in urine (Ur) samples were studied using QRT-PCR. Ninety-five KTP and 111 Ur samples were evaluated. Patients (Pts) were divided as, within six months (N = 31), and with more than six months post-KT (N = 64), KTP with more than six months post-KT were classified as KTP with stable kidney function (SKF) (N = 32), KTP with SKF (creatinine < 2 mg/dL) and proteinuria > 500 mg/24 h (N = 18), and KTP with biopsy proven CAN (N = 14). F-test was used to test for equality of variances between groups. IL-10 mRNA was decreased in Ur samples from KTP with less than six months post-KT (P = 0.005). For KTR groups with more than six months post-KT, AGT and EGFR mRNA were statistically different among KTP with SKF, KTP with SKF and proteinuria, and CAN Pts (P = 0.003, and P = 0.01), with KTP with SKF having higher mean expression. TSP-1 mRNA levels also were significantly different among these three groups (P = 0.04), with higher expression observed in CAN Pts. Using the random forest algorithm, AGT, EGFR, and TGF-β1 were identified as predictors of CAN, SKF, SKF with proteinuria. A characteristic pattern of mRNA levels in the different KTP groups was observed indicating that the mRNA levels in Ur cells might reflect allograft function.",
author = "Valeria Mas and Mas, {Luciana A.} and Archer, {Kellie J.} and Kenneth Yanek and King, {Anne L.} and Gibney, {Eric M.} and Adrian Cotterell and Fisher, {Robert A.} and Marc Posner and Daniel Maluf",
year = "2007",
month = "5",
day = "1",
doi = "10.2119/2007-00017.Mas",
language = "English (US)",
volume = "13",
pages = "315--324",
journal = "Molecular Medicine",
issn = "1076-1551",
publisher = "Feinstein Institute for Medical Research",
number = "5-6",

}

TY - JOUR

T1 - Evaluation of gene panel mRNAs in urine samples of kidney transplant recipients as a non-invasive tool of graft function

AU - Mas, Valeria

AU - Mas, Luciana A.

AU - Archer, Kellie J.

AU - Yanek, Kenneth

AU - King, Anne L.

AU - Gibney, Eric M.

AU - Cotterell, Adrian

AU - Fisher, Robert A.

AU - Posner, Marc

AU - Maluf, Daniel

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Non-invasive monitoring may be useful after kidney transplantation (KT), particularly for predicting acute rejection (AR). It is less clear whether chronic allograft nephropathy (CAN) is also associated with changes in urine cells. To identify non-invasive markers of allograft function in kidney transplant patients (KTP), mRNA levels of AGT, TGF-β1, EGFR, IFN-γ, TSP-1, and IL-10 in urine (Ur) samples were studied using QRT-PCR. Ninety-five KTP and 111 Ur samples were evaluated. Patients (Pts) were divided as, within six months (N = 31), and with more than six months post-KT (N = 64), KTP with more than six months post-KT were classified as KTP with stable kidney function (SKF) (N = 32), KTP with SKF (creatinine < 2 mg/dL) and proteinuria > 500 mg/24 h (N = 18), and KTP with biopsy proven CAN (N = 14). F-test was used to test for equality of variances between groups. IL-10 mRNA was decreased in Ur samples from KTP with less than six months post-KT (P = 0.005). For KTR groups with more than six months post-KT, AGT and EGFR mRNA were statistically different among KTP with SKF, KTP with SKF and proteinuria, and CAN Pts (P = 0.003, and P = 0.01), with KTP with SKF having higher mean expression. TSP-1 mRNA levels also were significantly different among these three groups (P = 0.04), with higher expression observed in CAN Pts. Using the random forest algorithm, AGT, EGFR, and TGF-β1 were identified as predictors of CAN, SKF, SKF with proteinuria. A characteristic pattern of mRNA levels in the different KTP groups was observed indicating that the mRNA levels in Ur cells might reflect allograft function.

AB - Non-invasive monitoring may be useful after kidney transplantation (KT), particularly for predicting acute rejection (AR). It is less clear whether chronic allograft nephropathy (CAN) is also associated with changes in urine cells. To identify non-invasive markers of allograft function in kidney transplant patients (KTP), mRNA levels of AGT, TGF-β1, EGFR, IFN-γ, TSP-1, and IL-10 in urine (Ur) samples were studied using QRT-PCR. Ninety-five KTP and 111 Ur samples were evaluated. Patients (Pts) were divided as, within six months (N = 31), and with more than six months post-KT (N = 64), KTP with more than six months post-KT were classified as KTP with stable kidney function (SKF) (N = 32), KTP with SKF (creatinine < 2 mg/dL) and proteinuria > 500 mg/24 h (N = 18), and KTP with biopsy proven CAN (N = 14). F-test was used to test for equality of variances between groups. IL-10 mRNA was decreased in Ur samples from KTP with less than six months post-KT (P = 0.005). For KTR groups with more than six months post-KT, AGT and EGFR mRNA were statistically different among KTP with SKF, KTP with SKF and proteinuria, and CAN Pts (P = 0.003, and P = 0.01), with KTP with SKF having higher mean expression. TSP-1 mRNA levels also were significantly different among these three groups (P = 0.04), with higher expression observed in CAN Pts. Using the random forest algorithm, AGT, EGFR, and TGF-β1 were identified as predictors of CAN, SKF, SKF with proteinuria. A characteristic pattern of mRNA levels in the different KTP groups was observed indicating that the mRNA levels in Ur cells might reflect allograft function.

UR - http://www.scopus.com/inward/record.url?scp=34547396868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547396868&partnerID=8YFLogxK

U2 - 10.2119/2007-00017.Mas

DO - 10.2119/2007-00017.Mas

M3 - Article

VL - 13

SP - 315

EP - 324

JO - Molecular Medicine

JF - Molecular Medicine

SN - 1076-1551

IS - 5-6

ER -