Evaluation of zeta-potential and particle size of technetium 99mTc-sulfur colloid subsequent to the addition of lidocaine and sodium bicarbonate

Vivian Loveless, Christopher P. Surdock, Himanshu Bhattacharjee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The use of 99mTc-sulfur colloid lymphoscintigraphy for the determination of lymph flow patterns from a tumor site and localization of the sentinel node has been widely adopted. However, the effects of multiple injections of the radiopharmaceutical can range from mild discomfort to pain. pH-adjusted lidocaine HCl coadministered with 99mTc-sulfur colloid presents a risk of introducing instability of the radiopharmaceutical, which could lead to aggregation, possibly impeding the kinetics of lymphatic drainage from the tumor site. Methods: In the present study, lidocaine pH-adjusted with 4.2%, 6.3%, or 8.4% sodium bicarbonate was added to the 99mTc-sulfur colloid radiopharmaceutical to monitor effects on radiochemical purity, zeta-potential, particle size, and pH. These parameters were then used to evaluate the shortterm stability of the preparation. Results: The study revealed that the formulation of lidocaine pH-adjusted with 8.4% sodium bicarbonate added to 99mTc-sulfur colloid demonstrated a similar change in zeta-potential (-4.09 ± 2.90 mV) and particle size (10-330 nm) to that of control filtered 99mTc-sulfur colloid (-5.09 ± 1.68 mV and 11-343 nm, respectively). However, the 4.2% preparation showed a zeta-potential of -3.01 ± 2.24 mV and a particle size range of 10-351 nm. The pH of the 8.4% buffered preparation, at 7.1, was closer to physiologic pH than was the control, at 6.0. The 6.3% pH-adjusted lidocaine- 99mTc-sulfur colloid preparation failed radiochemical purity; thus, it was not included in the analysis. Conclusion: Compared with other 99mTc-sulfur colloid test formulations of 4.2% and 6.3% pHadjusted lidocaine, the 8.4% sodium bicarbonate pH-adjusted lidocaine- 99mTc-sulfur colloid preparation, taken as a whole, yielded superior quality-control parameters. This formulation would be an acceptable alternative to the control.

Original languageEnglish (US)
Pages (from-to)49-52
Number of pages4
JournalJournal of Nuclear Medicine Technology
Volume38
Issue number1
DOIs
StatePublished - Mar 1 2010

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Technetium Tc 99m Sulfur Colloid
Sodium Bicarbonate
Colloids
Lidocaine
Particle Size
Sulfur
Radiopharmaceuticals
Lymphoscintigraphy
Lymph
Quality Control
Drainage
Neoplasms
Pain
Injections

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Evaluation of zeta-potential and particle size of technetium 99mTc-sulfur colloid subsequent to the addition of lidocaine and sodium bicarbonate. / Loveless, Vivian; Surdock, Christopher P.; Bhattacharjee, Himanshu.

In: Journal of Nuclear Medicine Technology, Vol. 38, No. 1, 01.03.2010, p. 49-52.

Research output: Contribution to journalArticle

@article{648064f64f344458a5f6b0c19585964c,
title = "Evaluation of zeta-potential and particle size of technetium 99mTc-sulfur colloid subsequent to the addition of lidocaine and sodium bicarbonate",
abstract = "The use of 99mTc-sulfur colloid lymphoscintigraphy for the determination of lymph flow patterns from a tumor site and localization of the sentinel node has been widely adopted. However, the effects of multiple injections of the radiopharmaceutical can range from mild discomfort to pain. pH-adjusted lidocaine HCl coadministered with 99mTc-sulfur colloid presents a risk of introducing instability of the radiopharmaceutical, which could lead to aggregation, possibly impeding the kinetics of lymphatic drainage from the tumor site. Methods: In the present study, lidocaine pH-adjusted with 4.2{\%}, 6.3{\%}, or 8.4{\%} sodium bicarbonate was added to the 99mTc-sulfur colloid radiopharmaceutical to monitor effects on radiochemical purity, zeta-potential, particle size, and pH. These parameters were then used to evaluate the shortterm stability of the preparation. Results: The study revealed that the formulation of lidocaine pH-adjusted with 8.4{\%} sodium bicarbonate added to 99mTc-sulfur colloid demonstrated a similar change in zeta-potential (-4.09 ± 2.90 mV) and particle size (10-330 nm) to that of control filtered 99mTc-sulfur colloid (-5.09 ± 1.68 mV and 11-343 nm, respectively). However, the 4.2{\%} preparation showed a zeta-potential of -3.01 ± 2.24 mV and a particle size range of 10-351 nm. The pH of the 8.4{\%} buffered preparation, at 7.1, was closer to physiologic pH than was the control, at 6.0. The 6.3{\%} pH-adjusted lidocaine- 99mTc-sulfur colloid preparation failed radiochemical purity; thus, it was not included in the analysis. Conclusion: Compared with other 99mTc-sulfur colloid test formulations of 4.2{\%} and 6.3{\%} pHadjusted lidocaine, the 8.4{\%} sodium bicarbonate pH-adjusted lidocaine- 99mTc-sulfur colloid preparation, taken as a whole, yielded superior quality-control parameters. This formulation would be an acceptable alternative to the control.",
author = "Vivian Loveless and Surdock, {Christopher P.} and Himanshu Bhattacharjee",
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T1 - Evaluation of zeta-potential and particle size of technetium 99mTc-sulfur colloid subsequent to the addition of lidocaine and sodium bicarbonate

AU - Loveless, Vivian

AU - Surdock, Christopher P.

AU - Bhattacharjee, Himanshu

PY - 2010/3/1

Y1 - 2010/3/1

N2 - The use of 99mTc-sulfur colloid lymphoscintigraphy for the determination of lymph flow patterns from a tumor site and localization of the sentinel node has been widely adopted. However, the effects of multiple injections of the radiopharmaceutical can range from mild discomfort to pain. pH-adjusted lidocaine HCl coadministered with 99mTc-sulfur colloid presents a risk of introducing instability of the radiopharmaceutical, which could lead to aggregation, possibly impeding the kinetics of lymphatic drainage from the tumor site. Methods: In the present study, lidocaine pH-adjusted with 4.2%, 6.3%, or 8.4% sodium bicarbonate was added to the 99mTc-sulfur colloid radiopharmaceutical to monitor effects on radiochemical purity, zeta-potential, particle size, and pH. These parameters were then used to evaluate the shortterm stability of the preparation. Results: The study revealed that the formulation of lidocaine pH-adjusted with 8.4% sodium bicarbonate added to 99mTc-sulfur colloid demonstrated a similar change in zeta-potential (-4.09 ± 2.90 mV) and particle size (10-330 nm) to that of control filtered 99mTc-sulfur colloid (-5.09 ± 1.68 mV and 11-343 nm, respectively). However, the 4.2% preparation showed a zeta-potential of -3.01 ± 2.24 mV and a particle size range of 10-351 nm. The pH of the 8.4% buffered preparation, at 7.1, was closer to physiologic pH than was the control, at 6.0. The 6.3% pH-adjusted lidocaine- 99mTc-sulfur colloid preparation failed radiochemical purity; thus, it was not included in the analysis. Conclusion: Compared with other 99mTc-sulfur colloid test formulations of 4.2% and 6.3% pHadjusted lidocaine, the 8.4% sodium bicarbonate pH-adjusted lidocaine- 99mTc-sulfur colloid preparation, taken as a whole, yielded superior quality-control parameters. This formulation would be an acceptable alternative to the control.

AB - The use of 99mTc-sulfur colloid lymphoscintigraphy for the determination of lymph flow patterns from a tumor site and localization of the sentinel node has been widely adopted. However, the effects of multiple injections of the radiopharmaceutical can range from mild discomfort to pain. pH-adjusted lidocaine HCl coadministered with 99mTc-sulfur colloid presents a risk of introducing instability of the radiopharmaceutical, which could lead to aggregation, possibly impeding the kinetics of lymphatic drainage from the tumor site. Methods: In the present study, lidocaine pH-adjusted with 4.2%, 6.3%, or 8.4% sodium bicarbonate was added to the 99mTc-sulfur colloid radiopharmaceutical to monitor effects on radiochemical purity, zeta-potential, particle size, and pH. These parameters were then used to evaluate the shortterm stability of the preparation. Results: The study revealed that the formulation of lidocaine pH-adjusted with 8.4% sodium bicarbonate added to 99mTc-sulfur colloid demonstrated a similar change in zeta-potential (-4.09 ± 2.90 mV) and particle size (10-330 nm) to that of control filtered 99mTc-sulfur colloid (-5.09 ± 1.68 mV and 11-343 nm, respectively). However, the 4.2% preparation showed a zeta-potential of -3.01 ± 2.24 mV and a particle size range of 10-351 nm. The pH of the 8.4% buffered preparation, at 7.1, was closer to physiologic pH than was the control, at 6.0. The 6.3% pH-adjusted lidocaine- 99mTc-sulfur colloid preparation failed radiochemical purity; thus, it was not included in the analysis. Conclusion: Compared with other 99mTc-sulfur colloid test formulations of 4.2% and 6.3% pHadjusted lidocaine, the 8.4% sodium bicarbonate pH-adjusted lidocaine- 99mTc-sulfur colloid preparation, taken as a whole, yielded superior quality-control parameters. This formulation would be an acceptable alternative to the control.

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