Evidence for a role of taurine in the in vitro oxidative toxicity of neutrophils toward erythrocytes

Edwin Thomas, M. B. Grisham, D. F. Melton, M. M. Jefferson

Research output: Contribution to journalArticle

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Abstract

Production of hydrogen peroxide and secretion of myeloperoxidase by stimulated neutrophils resulted in myeloperoxidase-catalyzed oxidation of chloride to hypochlorous acid (HOCl), the reaction of HOCl with taurine to yield taurine monochloramine (TauNHCl), and accumulation of TauNHCl in the extracellular medium. When erythrocytes were present, the yield of TauNHCl was lower as the result of uptake of TauNHCl into erythrocytes. The zwitterion taurine was not taken up, but the anion Tau NHCl and other anionic oxidants including taurine dichloramine (TauNCl2) and L-alanine chloramines were transported into erythrocytes by the anion-transport system. Oxidation of intracellular components such as glutathione (GSH) by taurine chloramines resulted in reduction of the chloramines and trapping of taurine within erythrocytes. At high oxidant:erythrocyte ratios, TauNHCl also oxidized hemoglobin (Hb) and depleted ATP, but caused little lysis. TauNCl2 was much more effective as a lytic agent. At low oxidant:erythrocyte ratios, the chloramines caused net loss of GSH when no glucose was provided, but Hb was not oxidized and GSH content returned to normal when glucose was added. Therefore, anionic chloramines may mediate oxidative toxicity when the neutrophil:erythrocyte ratio is high. Under more physiologic conditions, chlorination of taurine by neutrophils by neutrophils and the uptake and reduction of TauNHCl by erythrocytes prevents accumulation of oxidants and may protect blood cells, plasma components, and tissues against oxidative toxicity.

Original languageEnglish (US)
Pages (from-to)3321-3329
Number of pages9
JournalJournal of Biological Chemistry
Volume260
Issue number6
StatePublished - 1985
Externally publishedYes

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Chloramines
Taurine
Oxidants
Toxicity
Neutrophils
Erythrocytes
Hypochlorous Acid
Peroxidase
Anions
Hemoglobins
Glucose
Oxidation
Chlorination
Alanine
Hydrogen Peroxide
Glutathione
Chlorides
Blood
Adenosine Triphosphate
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Evidence for a role of taurine in the in vitro oxidative toxicity of neutrophils toward erythrocytes. / Thomas, Edwin; Grisham, M. B.; Melton, D. F.; Jefferson, M. M.

In: Journal of Biological Chemistry, Vol. 260, No. 6, 1985, p. 3321-3329.

Research output: Contribution to journalArticle

Thomas, Edwin ; Grisham, M. B. ; Melton, D. F. ; Jefferson, M. M. / Evidence for a role of taurine in the in vitro oxidative toxicity of neutrophils toward erythrocytes. In: Journal of Biological Chemistry. 1985 ; Vol. 260, No. 6. pp. 3321-3329.
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